Xu Kang, Al-Ani Mohanad Kh, Sun Yanjun, Xu Wei, Pan Lianhong, Song Yang, Xu ZhiLing, Pan Xin, Yang Li
National Innovation and Attracting Talents '111' Base, Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, People's Republic of China.
Veterinary College, Tikrit University, Iraq.
J Tissue Eng Regen Med. 2017 Apr;11(4):1173-1184. doi: 10.1002/term.2020. Epub 2015 Mar 11.
This study investigates whether platelet-rich plasma (PRP) is an activator of tendon-derived stem cells (TDSCs) to promote regeneration of Achilles tendon post-rupture in rats. In the in vitro study, PRGF (activated PRP) significantly enhanced cell DNA synthesis, improved viability and promoted proliferation, while facilitating cell migration and the recruitment of TDSCs. In addition, TDSCs were mixed with collagen and PRP to form collagen-TDSC constructs (CTC) and PRP-collagen-TDSC constructs (PCTCs). After 3 weeks of culture in vitro, we found that most of the encapsulated TDSCs in the CTCs and PCTCs were still alive, while cells in the PCTCs showed a more aligned arrangement compared to the CTCs. In addition, the micro-structure of PCTC showed more obvious fibre-like tissues and formed a cyclic microvascular structure. The tenocyte-related genes types I and III collagen, Tenascin-C and Scleraxis of TDSCs in the PCTCs and CTCs were upregulated with time, and PCTCs showed more significance than CTCs (p < 0.05). After in vivo transplantation, the CTCs and PCTCs showed stimulatory effects on Achilles tendon healing. Moreover, the PCTCs improved the macroscopic appearance, histological morphology and biomechanical strength of ruptured Achilles tendon better than CTC. These results indicate that PRP can activate TDSCs to improve the quality of Achilles tendon rupture healing in the early stages. Copyright © 2015 John Wiley & Sons, Ltd.
本研究旨在探究富血小板血浆（PRP）是否为肌腱来源干细胞（TDSCs）的激活剂，以促进大鼠跟腱断裂后的再生。在体外研究中，PRGF（活化的PRP）显著增强细胞DNA合成，提高细胞活力并促进增殖，同时促进细胞迁移以及TDSCs的募集。此外，将TDSCs与胶原蛋白和PRP混合，形成胶原蛋白-TDSC构建体（CTC）和PRP-胶原蛋白-TDSC构建体（PCTC）。体外培养3周后，我们发现CTC和PCTC中大多数被包裹的TDSCs仍然存活，而与CTC相比，PCTC中的细胞排列更整齐。此外，PCTC的微观结构显示出更明显的纤维状组织，并形成了环状微血管结构。随着时间的推移，PCTC和CTC中TDSCs的I型和III型胶原蛋白、肌腱蛋白-C和硬骨素等肌腱细胞相关基因上调，且PCTC比CTC更显著（p<0.05）。体内移植后，CTC和PCTC对跟腱愈合均有刺激作用。此外，PCTC在改善跟腱断裂后的宏观外观、组织形态学和生物力学强度方面优于CTC。这些结果表明，PRP可激活TDSCs，以改善早期跟腱断裂愈合的质量。版权所有©2015约翰威立父子有限公司。
