Department of Orthopaedics Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
J Orthop Res. 2012 Jun;30(6):991-7. doi: 10.1002/jor.22033. Epub 2011 Dec 12.
Injured rat Achilles tendons were treated with botulism toxin to create a mechanically unloaded condition (unloaded) or left untreated (loaded), and then treated with phosphate-buffered saline (PBS), platelet-rich plasma (PRP), tendon stem cells (TSCs), or a combination (TSCs + PRP). mRNA and protein expression of collagen I, collagen III, tenascin C, and Smad 8 were determined by real time PCR and immunostaining, respectively. Loaded tendons treated with PBS, PRP, or TSCs for 3 or 14 days had higher collagen I mRNA expression than unloaded tendons. Loaded tendons treated with PBS for 3 or 14 days or with PRP for 3 days had higher collagen I protein levels than unloaded tendons. Loaded tendons treated for 3 days with PBS, for 14 days with PRP or TSCs or TSCs + PRP for 3 or 14 days had higher collagen III protein levels than unloaded tendons. Collagen I mRNA levels were higher in TSCs + PRP-treated loaded tendons compared to PBS-treated loaded tendons on day 3 of treatment. Based on changes in the expression of tendon-healing genes, our data suggest that the combination of TSCs and PRP has synergistic effects on tendon healing under both loaded and unloaded conditions, and loaded conditions improve tendon healing.
受伤的大鼠跟腱用肉毒杆菌毒素处理以产生机械卸载条件（卸载）或未经处理（加载），然后用磷酸盐缓冲盐水（PBS）、富含血小板的血浆（PRP）、肌腱干细胞（TSCs）或组合（TSCs+PRP）处理。通过实时 PCR 和免疫染色分别测定胶原 I、胶原 III、腱糖蛋白 C 和 Smad8 的 mRNA 和蛋白表达。加载的肌腱用 PBS、PRP 或 TSCs 处理 3 或 14 天的胶原 I mRNA 表达高于未加载的肌腱。加载的肌腱用 PBS 处理 3 或 14 天或 PRP 处理 3 天的胶原 I 蛋白水平高于未加载的肌腱。加载的肌腱用 PBS 处理 3 天，用 PRP 或 TSCs 处理 14 天，用 TSCs+PRP 处理 3 或 14 天的胶原 III 蛋白水平高于未加载的肌腱。在治疗的第 3 天，TSCs+PRP 处理的加载肌腱中的胶原 I mRNA 水平高于 PBS 处理的加载肌腱。根据肌腱愈合基因表达的变化，我们的数据表明，TSCs 和 PRP 的组合在加载和未加载条件下对肌腱愈合均具有协同作用，并且加载条件可改善肌腱愈合。
