Guasconi Lorena, Chiapello Laura S, Masih Diana T
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba 5000, Argentina.
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Medina Allende y Haya de la Torre, Ciudad Universitaria, Córdoba 5000, Argentina.
Immunobiology. 2015 Jul;220(7):934-9. doi: 10.1016/j.imbio.2015.02.001. Epub 2015 Feb 25.
Fasciola hepatica excretory-secretory products (FhESP) induce immunomodulatory effects on macrophages. Previously, we demonstrated that these effects are dependent on Dectin-1. Therefore, the aim of this study was to determine how this affects the CD4 T-cells immune response. We observed that FhESP induce an increased expression of PD-L2 in macrophages via Dectin-1. Furthermore, in co-cultures with CD4 T-cell we observed a suppressive effect on proliferative response, down-modulation of IFN-γ and up-modulation of IL-10 via Dectin-1 on macrophages. These results suggest that FhESP induce T-cell anergy via selective up-regulation of PD-L2 expression on macrophages in a Dectin-1 dependent way.
肝片吸虫排泄分泌产物（FhESP）对巨噬细胞具有免疫调节作用。此前，我们证明这些作用依赖于树突状细胞相关C型凝集素-1（Dectin-1）。因此，本研究的目的是确定这如何影响CD4 T细胞免疫反应。我们观察到FhESP通过Dectin-1诱导巨噬细胞中程序性死亡配体2（PD-L2）表达增加。此外，在与CD4 T细胞的共培养中，我们观察到通过Dectin-1对巨噬细胞的增殖反应有抑制作用、对γ干扰素（IFN-γ）有下调作用以及对白细胞介素-10（IL-10）有上调作用。这些结果表明，FhESP通过以Dectin-1依赖的方式选择性上调巨噬细胞上PD-L2的表达来诱导T细胞无能。
