Department of Research and Development, Bio Ray Biotech Inc., 3F., No. 466, Bo'ai 1st Road, Gushan District, Kaohsiung City 80466, Taiwan.
J Immunol Res. 2014;2014:721505. doi: 10.1155/2014/721505. Epub 2014 Dec 11.
A personalized probiotic microfluidic chip system has been established and used to screen the probiotics which had the highest value of IFN-γ/IL-10 or IL-10/IFN-γ among six probiotics, including L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500, and L. reuteri BR101. One hundred volunteers were included and their PBMCs were collected and stimulated by the six probiotics. People who belonged to the IFN-γ group took the probiotics that exerted the highest ratio of IFN-γ/IL-10 and vice versa in IL-10 group. A significant increase in NK cytotoxicity of 69 volunteers in the IFN-γ group was observed compared to the IL-10 group (n = 21) and control group (n = 10). The result also showed that L. paracasei BRAP01 and L. acidophilus AD300 were the two dominant inducers in IFN-γ group which yielded higher value of IFN-γ/IL-10 than the other 4 probiotics, while L. reuteri BR101 was the most effective agent on the ratio of IL-10/IFN-γ in the IL-10 group. Our finding highlighted the concept of personalized probiotics and also provided a good foundation to investigate the probiotics with NK activity.
已经建立了一种个性化益生菌微流控芯片系统，并用于筛选六种益生菌中 IFN-γ/IL-10 或 IL-10/IFN-γ 值最高的益生菌，包括 L. paracasei BRAP01、L. acidophilus AD300、B. longum BA100、E. faecium BR0085、L. rhamnosus AD500 和 L. reuteri BR101。纳入了 100 名志愿者，收集他们的 PBMC 并由这六种益生菌刺激。属于 IFN-γ 组的人服用了 IFN-γ/IL-10 比值最高的益生菌，而 IL-10 组则相反。与 IL-10 组（n=21）和对照组（n=10）相比，IFN-γ 组的 69 名志愿者的 NK 细胞毒性显著增加。结果还表明，L. paracasei BRAP01 和 L. acidophilus AD300 是 IFN-γ 组中两种主要的诱导剂，其 IFN-γ/IL-10 比值高于其他 4 种益生菌，而 L. reuteri BR101 是 IL-10 组中 IL-10/IFN-γ 比值最有效的制剂。我们的发现强调了个性化益生菌的概念，并为研究具有 NK 活性的益生菌提供了良好的基础。
