Miao W, Bao T H, Han J H, Yin M, Yan Y, Wang W W, Zhu Y H
Department of Neurology, Second Affiliated Hospital, Kunming Medical University, Kunming, China.
Department of Cardiology, Second Affiliated Hospital, Kunming Medical University, Kunming, China.
Braz J Med Biol Res. 2015 May;48(5):433-9. doi: 10.1590/1414-431X20144012. Epub 2015 Mar 6.
MicroRNAs (miRNAs) may be important mediators of the profound molecular and cellular changes that occur after traumatic brain injury (TBI). However, the changes and possible roles of miRNAs induced by voluntary exercise prior to TBI are still not known. In this report, the microarray method was used to demonstrate alterations in miRNA expression levels in the cerebral cortex of TBI mice that were pretrained on a running wheel (RW). Voluntary RW exercise prior to TBI: i) significantly decreased the mortality rate and improved the recovery of the righting reflex in TBI mice, and ii) differentially changed the levels of several miRNAs, upregulating some and downregulating others. Furthermore, we revealed global upregulation of miR-21, miR-92a, and miR-874 and downregulation of miR-138, let-7c, and miR-124 expression among the sham-non-runner, TBI-non-runner, and TBI-runner groups. Quantitative reverse transcription polymerase chain reaction data (RT-qPCR) indicated good consistency with the microarray results. Our microarray-based analysis of miRNA expression in mice cerebral cortex after TBI revealed that some miRNAs such as miR-21, miR-92a, miR-874, miR-138, let-7c, and miR-124 could be involved in the prevention and protection afforded by voluntary exercise in a TBI model.
微小RNA（miRNA）可能是创伤性脑损伤（TBI）后发生的深刻分子和细胞变化的重要介质。然而，TBI之前自愿运动诱导的miRNA的变化及其可能的作用仍不清楚。在本报告中，使用微阵列方法来证明在跑步轮（RW）上进行预训练的TBI小鼠大脑皮质中miRNA表达水平的变化。TBI之前的自愿RW运动：i）显著降低了TBI小鼠的死亡率并改善了翻正反射的恢复，并且ii）差异改变了几种miRNA的水平，上调了一些miRNA而下调了其他miRNA。此外，我们发现在假手术-未跑步组、TBI-未跑步组和TBI-跑步组中，miR-21、miR-92a和miR-874全局上调，而miR-138、let-7c和miR-124表达下调。定量逆转录聚合酶链反应数据（RT-qPCR）表明与微阵列结果具有良好的一致性。我们基于微阵列对TBI后小鼠大脑皮质中miRNA表达的分析表明，一些miRNA，如miR-21、miR-92a、miR-874、miR-138、let-7c和miR-124可能参与了TBI模型中自愿运动提供的预防和保护作用。
