Lang J W, Aggarwal R K, Majumdar K C, Singh L
Department of Biology, University of North Dakota, Grand Forks.
Mol Gen Genet. 1993 Apr;238(1-2):49-58. doi: 10.1007/BF00279530.
Individual-specific DNA fingerprints of crocodilians were obtained by the use of Bkm-2(8) probe. Pedigree analyses of Crocodylus palustris, C. porosus and Caiman crocodilus revealed that the multiple bands (22-23 bands with Aludigest) thus obtained were inherited stably in a Mendelian fashion. Unique fingerprints permitted us to identify individuals, assign parentage, and reconstruct the DNA profile of a missing parent. Average band sharing between unrelated crocodiles was found to be 0.37. Band sharing between animals of known pedigrees increased predictably with relatedness and provided a basis for distinguishing relatives from non-relatives. Similar results obtained in other species/genera, using the same probe, suggest that this approach may be applicable to all species of crocodilians, and could facilitate genetic studies of wild and captive populations.
通过使用Bkm - 2(8)探针获得了鳄类动物个体特异性的DNA指纹图谱。对泽鳄、湾鳄和凯门鳄进行的谱系分析表明，由此获得的多条带（用Alu消化后有22 - 23条带）以孟德尔方式稳定遗传。独特的指纹图谱使我们能够识别个体、确定亲子关系并重建缺失亲本的DNA图谱。发现不相关鳄鱼之间的平均条带共享率为0.37。已知谱系的动物之间的条带共享率随亲缘关系增加而可预测地增加，为区分亲属和非亲属提供了依据。使用相同探针在其他物种/属中获得的类似结果表明，这种方法可能适用于所有鳄类物种，并有助于对野生和圈养种群进行遗传研究。
