Metzler-Zebeli Barbara U, Mann Evelyne, Ertl Reinhard, Schmitz-Esser Stephan, Wagner Martin, Klein Dieter, Ritzmann Mathias, Zebeli Qendrim
University Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna,Veterinärplatz 1,1210Vienna,Austria.
Research Cluster 'Animal Gut Health', Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna,Vienna,Austria.
Br J Nutr. 2015 Apr 14;113(7):1019-31. doi: 10.1017/S0007114515000380. Epub 2015 Mar 12.
Ca plays an essential role in bone development; however, little is known about its effect on intestinal gene expression in juvenile animals. In the present study, thirty-two weaned pigs (9·5 (SEM 0·11) kg) were assigned to four diets that differed in Ca concentration (adequate v. high) and cereal composition (wheat-barley v. maize) to assess the jejunal and colonic gene expression of nutrient transporters, tight junction proteins, cytokines and pathogen-associated molecular patterns, nutrient digestibility, Ca balance and serum acute-phase response. To estimate the impact of mucosal bacteria on colonic gene expression, Spearman's correlations between colonic gene expression and bacterial abundance were computed. Faecal Ca excretion indicated that more Ca was available along the intestinal tract of the pigs fed high Ca diets as compared to the pigs fed adequate Ca diets (P> 0.05). High Ca diets decreased jejunal zonula occludens 1 (ZO1) and occludin (OCLN) expression, up-regulated jejunal expression of toll-like receptor 2 (TLR2) and down-regulated colonic GLUT2 expression as compared to the adequate Ca diets (P< 0.05). Dietary cereal composition up-regulated jejunal TLR2 expression and interacted (P= 0.021) with dietary Ca on colonic IL1B expression; high Ca concentration up-regulated IL1B expression with wheat-barley diets and down-regulated it with maize diets. Spearman's correlations (r> 0·35; P< 0·05) indicated an association between operational taxonomic units assigned to the phyla Bacteroidetes, Firmicutes and Proteobacteria and bacterial metabolites and mucosal gene expression in the colon. The present results indicate that high Ca diets have the potential to modify the jejunal and colonic mucosal gene expression response which, in turn, interacts with the composition of the basal diet and mucosa-associated bacteria in weaned pigs.
钙在骨骼发育中起着至关重要的作用；然而，关于其对幼年动物肠道基因表达的影响却知之甚少。在本研究中，32头断奶仔猪（体重9.5（标准误0.11）千克）被分配到四种钙浓度（充足与高钙）和谷物组成（小麦 - 大麦与玉米）不同的日粮中，以评估空肠和结肠中营养物质转运蛋白、紧密连接蛋白、细胞因子和病原体相关分子模式的基因表达、营养物质消化率、钙平衡及血清急性期反应。为了评估黏膜细菌对结肠基因表达的影响，计算了结肠基因表达与细菌丰度之间的斯皮尔曼相关性。粪便钙排泄表明，与饲喂充足钙日粮的猪相比，饲喂高钙日粮的猪肠道中可利用的钙更多（P>0.05）。与充足钙日粮相比，高钙日粮降低了空肠闭合蛋白1（ZO1）和闭合蛋白（OCLN）的表达，上调了空肠Toll样受体2（TLR2）的表达，并下调了结肠葡萄糖转运蛋白2（GLUT2）的表达（P<0.05）。日粮谷物组成上调了空肠TLR2的表达，并与日粮钙在结肠白细胞介素1β（IL1B）表达上存在交互作用（P = 0.021）；高钙浓度在小麦 - 大麦日粮中上调了IL1B的表达，而在玉米日粮中则下调了IL1B的表达。斯皮尔曼相关性（r>0.35；P<0.05）表明，在结肠中，属于拟杆菌门、厚壁菌门和变形菌门的操作分类单元与细菌代谢产物及黏膜基因表达之间存在关联。目前的结果表明，高钙日粮有可能改变空肠和结肠黏膜基因表达反应，而这反过来又与断奶仔猪基础日粮的组成及黏膜相关细菌相互作用。
