Rutley Nicholas, Twell David
Department of Biology, University of Leicester, Leicester, LE1 7RH, UK.
Plant Reprod. 2015 Jun;28(2):73-89. doi: 10.1007/s00497-015-0261-7. Epub 2015 Mar 12.
Overview of pollen transcriptome studies. Pollen development is driven by gene expression, and knowledge of the molecular events underlying this process has undergone a quantum leap in the last decade through studies of the transcriptome. Here, we outline historical evidence for male haploid gene expression and review the wealth of pollen transcriptome data now available. Knowledge of the transcriptional capacity of pollen has progressed from genetic studies to the direct analysis of RNA and from gene-by-gene studies to analyses on a genomic scale. Microarray and/or RNA-seq data can now be accessed for all phases and cell types of developing pollen encompassing 10 different angiosperms. These growing resources have accelerated research and will undoubtedly inspire new directions and the application of system-based research into the mechanisms that govern the development, function and evolution of angiosperm pollen.
花粉转录组研究综述。花粉发育由基因表达驱动，在过去十年中，通过转录组研究，我们对这一过程背后分子事件的认识有了巨大飞跃。在此，我们概述雄性单倍体基因表达的历史证据，并回顾目前可得的丰富花粉转录组数据。对花粉转录能力的认识已从遗传研究发展到对RNA的直接分析，从逐个基因的研究发展到基因组规模的分析。现在可以获取涵盖10种不同被子植物的发育中花粉所有阶段和细胞类型的微阵列和/或RNA测序数据。这些不断增加的资源加速了研究，无疑将激发新的方向，并推动基于系统的研究应用于控制被子植物花粉发育、功能和进化的机制。
