Ince Elif Unal, Ileri Talia, Dogu Figen, Ates Can, Cakmakli Hasan, Dalva Klara, Ikinciogullari Aydan, Uysal Zumrut, Ertem Mehmet
Department of Pediatric Hematology, Ankara University Faculty of Medicine, Cebeci-Ankara, Turkey.
Pediatr Transplant. 2015 Jun;19(4):385-90. doi: 10.1111/petr.12453. Epub 2015 Mar 12.
BM remains an important source of stem cells. The BM characteristics change with age but the estimation of CD34 calculation of one CD34+ cell per 100 nucleated cells is used for all donors including pediatric donors in the operating room before getting the actual CD34 count. In order to see whether this formula is applicable for pediatric donors, we designed a retrospective study to see the affect of the age and sex on the BM NCC, CD34 count, and CD34/NCC ratios. Ninety-eight BM collections from 91 related donors were evaluated retrospectively (median age: nine yr [1.5-54 yr]; M/F: 41/50). A significant negative correlation was found between the donor age and NCC (r = -0.229, p < 0.05), CD34 count (r = -0.563, p < 0.01), and CD34/NCC (r = -0.664, p < 0.01). The negative correlation for CD34 count and CD34/NCC persisted in female and male donor groups. When donors younger than 16 yr of age were compared with the older donor group, the median NCC, median CD34 count, and CD34/NCC were significantly lower in the older group (p < 0.01). Age and sex have to be taken into consideration to avoid unnecessary high-volume collections and increased operating room time in the younger donors.
骨髓仍然是干细胞的重要来源。骨髓特征会随年龄变化，但在获得实际的CD34计数之前，手术室中对所有供者（包括儿科供者）都采用每100个有核细胞中有一个CD34+细胞的CD34计算估计值。为了观察该公式是否适用于儿科供者，我们设计了一项回顾性研究，以观察年龄和性别对骨髓有核细胞计数（NCC）、CD34计数以及CD34/NCC比值的影响。对来自91名相关供者的98次骨髓采集进行了回顾性评估（中位年龄：9岁[1.5 - 54岁]；男/女：41/50）。发现供者年龄与NCC（r = -0.229，p < 0.05）、CD34计数（r = -0.563，p < 0.01）以及CD34/NCC（r = -0.664，p < 0.01）之间存在显著负相关。CD34计数和CD34/NCC的负相关在女性和男性供者组中均持续存在。将年龄小于16岁的供者与年龄较大的供者组进行比较时，年龄较大组的中位NCC、中位CD34计数和CD34/NCC显著更低（p < 0.01）。必须考虑年龄和性别，以避免在较年轻供者中进行不必要的大容量采集并增加手术室时间。
