Brustovetskiĭ N N, Amerkhanov Z G
Zh Evol Biokhim Fiziol. 1989 Nov-Dec;25(6):718-23.
Studies have been made on the permeability of the inner membrane of the liver mitochondria from hibernating and active ground squirrels for succinate, glutamate, hydroxybutyrate and inorganic phosphate. The permeability was calculated from the rate of mitochondrial swelling in 100 mM ammonium salts of the substrates and phosphate. It was shown that the rate of mitochondrial swelling in hibernating animals is 2--3 times lower than in active ones, being essentially identical in a solution of ammonium phosphate. It was concluded that the permeability of the inner mitochondrial membrane for the substrates decreases in hibernating animals, remaining unaffected for phosphate. Calcium-induced activation of membrane phospholipase A2 facilitates the transport of oxidative substrates into the mitochondria of hibernating ground squirrels, significant increase in the mitochondrial respiration being simultaneously observed. The data obtained suggest that inhibition of transport of oxidative substrates is one of the main factors which account for a low respiration rate in the mitochondria of hibernating animals.
对冬眠和活跃状态的地松鼠肝脏线粒体内膜对琥珀酸、谷氨酸、羟基丁酸和无机磷酸盐的通透性进行了研究。通透性是根据线粒体在底物和磷酸盐的100 mM铵盐中的肿胀速率计算得出的。结果表明，冬眠动物中线粒体肿胀的速率比活跃动物低2至3倍，在磷酸铵溶液中基本相同。得出的结论是，冬眠动物中线粒体内膜对底物的通透性降低，而对磷酸盐的通透性不受影响。钙诱导的膜磷脂酶A2激活促进了氧化底物向冬眠地松鼠线粒体的转运，同时观察到线粒体呼吸显著增加。获得的数据表明，氧化底物转运的抑制是导致冬眠动物线粒体呼吸速率较低的主要因素之一。
