Brustovetskiĭ N N, Gogvadze V G, Maevskiĭ E I
Nauchnye Doki Vyss Shkoly Biol Nauki. 1988(4):14-20.
The possible mechanisms in regulation of the respiration rate of mitochondria from liver of hibernating ground squirrels have been investigated. The inhibition of respiration has been shown to be mainly due to the inhibition of electron transfer to the respiratory chain from flavoproteins to cytochrome c. Calcium ions evoke the gradual increase in the respiration rate of mitochondria from liver of hibernating ground squirrels which is abolished by adding albumin, ruthenium red and sovcaine. The lower content of free fatty acids and decreased rate of the oxidation of exogenic NADH in the external pathway indicate the decrease in the activity of phospholipase A2 in mitochondria from liver of hibernating ground squirrels. The decreased calcium capacity of mitochondria indicates the higher sensitivity to calcium ions. A conclusion concerning the leading role of phospholipase A2 in Ca-induced activation of respiration of mitochondria from liver of hibernating ground squirrels is made.
对冬眠地松鼠肝脏线粒体呼吸速率调节的可能机制进行了研究。已表明呼吸抑制主要是由于从黄素蛋白到细胞色素c向呼吸链的电子传递受到抑制。钙离子可引起冬眠地松鼠肝脏线粒体呼吸速率逐渐增加，而添加白蛋白、钌红和索夫卡因可消除这种增加。游离脂肪酸含量较低以及外源性NADH在外部途径中的氧化速率降低表明冬眠地松鼠肝脏线粒体中磷脂酶A2的活性降低。线粒体钙容量降低表明对钙离子的敏感性较高。得出了关于磷脂酶A2在钙诱导的冬眠地松鼠肝脏线粒体呼吸激活中起主导作用的结论。
