Gao Jing, Wang Feng, Liu Yanhou, Cai Mingjun, Xu Haijiao, Jiang Junguang, Wang Hongda
1] State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, China [2] Graduate University of Chinese Academy of Sciences, Beijing, China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, China.
Sci Rep. 2015 Mar 12;5:9045. doi: 10.1038/srep09045.
Signal transducers and activators of transcription (STATs) can transduce cytokine signals and regulate gene expression. The cellular localization and nuclear trafficking of STAT1, a representative of the STAT family with multiple transcriptional functions, is tightly related with transcription process, which usually happens in the interphase of the cell cycle. However, these priority questions regarding STAT1 distribution and localization at the different cell-cycle stages remain unclear. By using direct stochastic optical reconstruction microscopy (dSTORM), we found that the nuclear expression level of STAT1 increased gradually as the cell cycle carried out, especially after EGF stimulation. Furthermore, STAT1 formed clusters in the whole cell during the cell cycle, with the size and the number of clusters also increasing significantly from G1 to G2 phase, suggesting that transcription and other cell-cycle related activities can promote STAT1 to form more and larger clusters for fast response to signals. Our work reveals that the cellular localization and clustering distribution of STAT1 are associated with the cell cycle, and further provides an insight into the mechanism of cell-cycle regulated STAT1 signal transduction.
信号转导子和转录激活子（STATs）能够转导细胞因子信号并调节基因表达。STAT1作为具有多种转录功能的STAT家族的代表，其细胞定位和核运输与转录过程密切相关，转录过程通常发生在细胞周期的间期。然而，关于STAT1在不同细胞周期阶段的分布和定位的这些重要问题仍不清楚。通过使用直接随机光学重建显微镜（dSTORM），我们发现随着细胞周期的进行，STAT1的核表达水平逐渐增加，尤其是在表皮生长因子（EGF）刺激后。此外，STAT1在细胞周期中在整个细胞内形成簇，从G1期到G2期，簇的大小和数量也显著增加，这表明转录和其他与细胞周期相关的活动可以促进STAT1形成越来越多且越来越大的簇，以便对信号快速响应。我们的工作揭示了STAT1的细胞定位和簇状分布与细胞周期相关，并进一步深入了解了细胞周期调控的STAT1信号转导机制。
