Cai Mingming, Zhang Jie, Guan Lili, Zhao Min
Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
J Mater Sci Mater Med. 2015 Mar;26(3):149. doi: 10.1007/s10856-015-5491-6. Epub 2015 Mar 13.
Amniotic membrane transplantation (AMT) is considered a substantial treatment option in the management of ocular surface disorders. However, several inherent drawbacks still remain. The present study devised a novel implantable composite biomaterial of fibrin glue-double layer Amniotic membrane (AM) and evaluated the biomechanical properties and effects on corneal surface reconstruction in alkali-burned rabbit model. Biomechanic parameters were calculated by an electronic universal testing machine. Corneal alkali burning was done in the right eyes of thirty rabbits, which were randomized into three groups of ten animals each. The eyes in group 1 underwent fibrin glue-double layer AMT, the eyes in group 2 underwent ordinary single layer AMT, and the eyes in group 3 (control group) did not undergo any surgical procedure. Healing of corneal epithelial defect, extent of corneal vascularization and corneal clarity were assessed and compared at two time points. One month after surgery, animals were killed and the eyes were processed for histopathology. The fibrin glue-double layer AM composites had more ideal biomechanical properties. In fibrin glue-double layer AM group, the rate of epithelial healing, vascularization inhibition and corneal clarity was significantly better than the other two groups. Novel fibrin glue-double layer AMT with corneal alkali burns is more effective and useful for ocular surface reconstruction and has great potential applications.
羊膜移植（AMT）被认为是治疗眼表疾病的一种重要治疗选择。然而，仍存在一些固有的缺点。本研究设计了一种新型的可植入复合生物材料——纤维蛋白胶-双层羊膜（AM），并在碱烧伤兔模型中评估了其生物力学性能以及对角膜表面重建的影响。通过电子万能试验机计算生物力学参数。对30只家兔的右眼进行角膜碱烧伤，将其随机分为三组，每组10只动物。第1组的眼睛接受纤维蛋白胶-双层羊膜移植，第2组的眼睛接受普通单层羊膜移植，第3组（对照组）不进行任何手术操作。在两个时间点评估并比较角膜上皮缺损的愈合情况、角膜血管化程度和角膜透明度。术后1个月，处死动物并对眼睛进行组织病理学处理。纤维蛋白胶-双层羊膜复合材料具有更理想的生物力学性能。在纤维蛋白胶-双层羊膜组中，上皮愈合率、血管化抑制率和角膜透明度明显优于其他两组。新型纤维蛋白胶-双层羊膜移植治疗角膜碱烧伤对眼表重建更有效且有用，具有巨大的潜在应用价值。
