Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China ; National Research Center of Bee Product Processing, Ministry of Agriculture, Beijing 100093, China.
Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Evid Based Complement Alternat Med. 2015;2015:587383. doi: 10.1155/2015/587383. Epub 2015 Feb 12.
The objective of the present study was to evaluate the inhibitory properties of various extracts of propolis on alpha-glucosidase from baker's yeast and mammalian intestine. Inhibitory activities of aqueous ethanol extracts of propolis were determined by using 4-nitrophenyl-D-glucopyranoside, sucrose and maltose as substrates, and acarbose as a positive reference. All extracts were significantly effective in inhibiting α-glucosidase from baker's yeast and rat intestinal sucrase in comparison with acarbose (P < 0.05). The 75% ethanol extracts of propolis (75% EEP) showed the highest inhibitory effect on α-glucosidase and sucrase and were a noncompetitive inhibition mode. 50% EEP, 95%, EEP and 100% EEP exhibited a mixed inhibition mode, while water extracts of propolis (WEP) and 25% EEP demonstrated a competitive inhibition mode. Furthermore, WEP presented the highest inhibitory activity against maltase. These results suggest that aqueous ethanol extracts of propolis may be used as nutraceuticals for the regulation of postprandial hyperglycemia.
本研究旨在评估各种蜂胶提取物对来自面包酵母和哺乳动物肠道的α-葡萄糖苷酶的抑制特性。通过使用 4-硝基苯-D-吡喃葡萄糖苷、蔗糖和麦芽糖作为底物，并以阿卡波糖作为阳性对照，来测定蜂胶的水乙醇提取物的抑制活性。与阿卡波糖相比（P<0.05），所有提取物均显著有效抑制来自面包酵母的α-葡萄糖苷酶和大鼠肠蔗糖酶。蜂胶的 75%乙醇提取物（75%EEP）对α-葡萄糖苷酶和蔗糖酶表现出最高的抑制作用，呈非竞争性抑制模式。50%EEP、95%EEP、EEP 和 100%EEP 表现出混合抑制模式，而蜂胶的水提取物（WEP）和 25%EEP 则呈竞争性抑制模式。此外，WEP 对麦芽糖酶表现出最高的抑制活性。这些结果表明，蜂胶的水乙醇提取物可用作调节餐后高血糖的营养保健品。
