Zhou Yusun, Zhou Tingting, Jin Hua, Jing Tao, Song Bin, Zhou Yikai, Mei Surong, Lee Yong-Ill
State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
Department of Chemistry, Changwon National University, Changwon 641-773, Republic of Korea; Central Laboratory, Yanbian University Hospital, Yanji, Jilin 133000, China.
Talanta. 2015 May;137:1-10. doi: 10.1016/j.talanta.2015.01.008. Epub 2015 Jan 14.
Magnetic molecularly imprinted polymers (MMIPs) were prepared based on surface molecular imprinting using erythromycin (ERY) as template molecule and Fe3O4 nanoparticles as support substrate. The MMIPs possessed high adsorption capacity of 94.1 mg/g for ERY and the imprinting factor was 11.9 indicating good imprinted effect for ERY. Selective evaluation demonstrated favorable selectivity of MMIPs for multiple macrolide antibiotics (MACs). Using MMIPs as adsorptive material, a rapid and convenient magnetic solid-phase extraction (MSPE) procedure was established for simultaneous and selective separation of six MACs in pork, fish and shrimp samples, then the MACs was subjected to high-performance liquid chromatography-ultraviolet (HPLC-UV) analysis. At different fortified concentrations, the extraction recoveries could reach 89.1% and the relative standard deviations were lower than 12.4%. Chromatogram revealed the response signals of MACs in spiked samples were greatly enhanced and matrix interferences were effectively eliminated after treatment with MSPE. The proposed MSPE procedure coupled with HPLC-UV realized selective and sensitive determination of multiple MACs in foodstuff samples.
以红霉素（ERY）为模板分子、Fe3O4纳米粒子为载体基质，通过表面分子印迹法制备了磁性分子印迹聚合物（MMIPs）。MMIPs对ERY具有94.1 mg/g的高吸附容量，印迹因子为11.9，表明对ERY具有良好的印迹效果。选择性评价表明MMIPs对多种大环内酯类抗生素（MACs）具有良好的选择性。以MMIPs为吸附材料，建立了一种快速简便的磁性固相萃取（MSPE）方法，用于同时选择性分离猪肉、鱼肉和虾肉样品中的6种MACs，然后对MACs进行高效液相色谱-紫外（HPLC-UV）分析。在不同的加标浓度下，萃取回收率可达89.1%，相对标准偏差低于12.4%。色谱图显示，经MSPE处理后，加标样品中MACs的响应信号显著增强，基质干扰得到有效消除。所提出的MSPE方法与HPLC-UV联用，实现了食品样品中多种MACs的选择性和灵敏测定。
