一种含有麻疹疫苗的微针贴片在非人灵长类动物中具有免疫原性。
A microneedle patch containing measles vaccine is immunogenic in non-human primates.
作者信息
Edens Chris, Collins Marcus L, Goodson James L, Rota Paul A, Prausnitz Mark R
机构信息
Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology, Atlanta, GA 30332, USA.
National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
出版信息
Vaccine. 2015 Sep 8;33(37):4712-8. doi: 10.1016/j.vaccine.2015.02.074. Epub 2015 Mar 12.
Very high vaccination coverage is required to eliminate measles, but achieving high coverage can be constrained by the logistical challenges associated with subcutaneous injection. To simplify the logistics of vaccine delivery, a patch containing micron-scale polymeric needles was formulated to encapsulate the standard dose of measles vaccine (1000 TCID₅₀) and the immunogenicity of the microneedle patch was compared with subcutaneous injection in rhesus macaques. The microneedle patch was administered without reconstitution with diluent, dissolved in skin within 10 min, and caused only mild, transient skin erythema. Both groups of rhesus macaques generated neutralizing antibody responses to measles that were consistent with protection and the neutralizing antibody titers were equivalent. In addition, the microneedle patches maintained an acceptable level of potency after storage at elevated temperature suggesting improved thermostability compared to standard lyophilized vaccine. In conclusion, a measles microneedle patch vaccine was immunogenic in non-human primates, and this approach offers a promising delivery method that could help increase vaccination coverage.
要消除麻疹需要非常高的疫苗接种覆盖率，但实现高覆盖率可能会受到与皮下注射相关的后勤挑战的限制。为了简化疫苗递送的后勤工作，制备了一种含有微米级聚合物针的贴片，用于封装标准剂量的麻疹疫苗（1000 TCID₅₀），并在恒河猴中将微针贴片的免疫原性与皮下注射进行了比较。微针贴片无需用稀释剂复溶即可给药，在10分钟内溶解于皮肤中，仅引起轻微的、短暂的皮肤红斑。两组恒河猴均产生了与保护作用一致的针对麻疹的中和抗体反应，且中和抗体滴度相当。此外，微针贴片在高温储存后保持了可接受的效价水平，表明与标准冻干疫苗相比，其热稳定性有所提高。总之，麻疹微针贴片疫苗在非人灵长类动物中具有免疫原性，这种方法提供了一种有前景的递送方式，有助于提高疫苗接种覆盖率。
Zotero 插件