Engström Patrik, Bergström Malin, Alfaro Astrid C, Syam Krishnan K, Bahnan Wael, Almqvist Fredrik, Bergström Sven
Department of Molecular Biology, Umeå University, SE-90187 Umeå, Sweden; Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, SE-90187 Umeå, Sweden; Umeå Centre for Microbial Research (UCMR), Umeå University, SE-90187 Umeå, Sweden; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA.
Department of Molecular Biology, Umeå University, SE-90187 Umeå, Sweden.
Int J Med Microbiol. 2015 May;305(3):378-82. doi: 10.1016/j.ijmm.2015.02.007. Epub 2015 Feb 27.
Chlamydia trachomatis replication takes place inside of a host cell, exclusively within a vacuole known as the inclusion. During an infection, the inclusion expands to accommodate the increasing numbers of C. trachomatis. However, whether inclusion expansion requires bacterial replication and/or de novo protein synthesis has not been previously investigated in detail. Therefore, using a chemical biology approach, we herein investigated C. trachomatis inclusion expansion under varying conditions in vitro. Under normal cell culture conditions, inclusion expansion correlated with C. trachomatis replication. When bacterial replication was inhibited using KSK120, an inhibitor that targets C. trachomatis glucose metabolism, inclusions expanded even in the absence of bacterial replication. In contrast, when bacterial protein synthesis was inhibited using chloramphenicol, expansion of inclusions was blocked. Together, these data suggest that de novo protein synthesis is necessary, whereas bacterial replication is dispensable for C. trachomatis inclusion expansion.
沙眼衣原体在宿主细胞内进行复制，且仅在一种称为包涵体的液泡内进行。在感染过程中，包涵体会扩大以容纳不断增多的沙眼衣原体。然而，包涵体的扩大是否需要细菌复制和/或从头合成蛋白质，此前尚未进行详细研究。因此，我们在此采用化学生物学方法，在体外不同条件下研究了沙眼衣原体包涵体的扩大情况。在正常细胞培养条件下，包涵体的扩大与沙眼衣原体的复制相关。当使用靶向沙眼衣原体葡萄糖代谢的抑制剂KSK120抑制细菌复制时，即使在没有细菌复制的情况下，包涵体仍会扩大。相反，当使用氯霉素抑制细菌蛋白质合成时，包涵体的扩大被阻断。这些数据共同表明，从头合成蛋白质是必要的，而细菌复制对于沙眼衣原体包涵体的扩大是可有可无的。
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