Alhalawani Adel M F, Rodriguez Omar, Curran Declan J, Co Russell, Kieran Sean, Arshad Saad, Keenan Timothy J, Wren Anthony W, Crasto Gazelle, Peel Sean A F, Towler Mark R
Department of Mechanical & Industrial Engineering, Faculty of Engineering and Architectural Science, Ryerson University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada.
J Mater Sci Mater Med. 2015 Mar;26(3):151. doi: 10.1007/s10856-015-5494-3. Epub 2015 Mar 14.
This work considers a glass polyalkenoate cement (GPC)-based carrier for the effective delivery of bone morphogenetic proteins (BMPs) at an implantation site. A 0.12 CaO-0.04 SrO-0.36 ZnO-0.48 SiO2 based glass and poly(acrylic acid) (PAA, Mw 213,000) were employed for the fabrication of the GPC. The media used for the water source in the GPC reaction was altered to produce a series of GPCs. The GPC liquid media was either 100 % distilled water with additions of albumin at 0, 2, 5 and 8 wt% of the glass content, 100 % formulation buffer (IFB), and 100 % BMP (150 µg rhBMP-2/ml IFB). Rheological properties, compressive strength, ion release profiles and BMP release were evaluated. Working times (Tw) of the formulated GPCs significantly increased with the addition of 2 % albumin and remained constant with further increases in albumin content or IFB solutions. Setting time (Ts) experienced an increase with 2 and 5 % albumin content, but a decrease with 8 % albumin. Changing the liquid source to IFB containing 5 % albumin had no significant effect on Ts compared to the 8 % albumin-containing BT101. Replacing the albumin with IFB/BMP-2 did not significantly affect Tw. However, Ts increased for the BT101_BMP-2 containing GPCs, compared to all other samples. The compressive strength evaluated 1 day post cement mixing was not affected significantly by the incorporation of BMPs, but the ion release did increase from the cements, particularly for Zn and Sr. The GPCs released BMP after the first day, which decreased in content during the following 6 days. This study has proven that BMPs can be immobilized into GPCs and may result in novel materials for clinical applications.
本研究探讨了一种基于玻璃聚烯烃酸酯水泥（GPC）的载体，用于在植入部位有效递送骨形态发生蛋白（BMP）。采用0.12CaO-0.04SrO-0.36ZnO-0.48SiO2基玻璃和聚（丙烯酸）（PAA，Mw 213,000）制备GPC。改变GPC反应中用作水源的介质，以制备一系列GPC。GPC液体介质为100%蒸馏水，并分别添加玻璃含量0、2、5和8 wt%的白蛋白、100%配方缓冲液（IFB）和100%BMP（150µg rhBMP-2/ml IFB）。评估了流变性能、抗压强度、离子释放曲线和BMP释放情况。添加2%白蛋白后，配制的GPC的工作时间（Tw）显著增加，随着白蛋白含量或IFB溶液的进一步增加，工作时间保持不变。凝固时间（Ts）在白蛋白含量为2%和5%时增加，但在白蛋白含量为8%时减少。与含8%白蛋白的BT101相比，将液体源改为含5%白蛋白的IFB对Ts没有显著影响。用IFB/BMP-2替代白蛋白对Tw没有显著影响。然而，与所有其他样品相比，含BT101_BMP-2的GPC的Ts增加。水泥混合后1天评估的抗压强度不受BMP掺入的显著影响，但水泥中的离子释放确实增加，特别是锌和锶。GPC在第一天后释放BMP，其含量在接下来的6天内下降。本研究证明，BMP可以固定在GPC中，并可能产生用于临床应用的新型材料。
