Hendijani Fatemeh, Javanmard Shaghayegh Haghjooy, Sadeghi-aliabadi Hojjat
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Tissue Cell. 2015 Jun;47(3):229-34. doi: 10.1016/j.tice.2015.01.005. Epub 2015 Jan 31.
Mesenchymal stem cell (MSC) therapy moves toward clinic progressively. Recent evidences establish anticancer effect of mesenchymal stem cells. However multiple factors including type of cancer, MSC source, study design, and animal model play role in final outcome. Wharton's jelly - a newly approved source of MSCs - possesses superiorities to bone marrow as the conventional source; therefore investigation of its medical effects can produce beneficial results. In this survey we examined cytotoxic and proapoptotic effect of human Wharton's jelly MSC secretome on K562 human leukemia cells. MSCs were isolated from human Wharton's jelly of umbilical cord by explant culture method, then characterized according to ISCT criteria (morphology and plastic adherence, surface antigenicity and differentiation potential). MSC secretome was collected and its cytotoxic and proapoptotic effects on K562 cells in combination with doxorubicin were evaluated using BrdU cell proliferation assay and Annexin V-PI staining. Our results showed antiproliferative effect of mesenchymal stem cell secretome on K562 cancer cells, the effect was also added to cytotoxic effect of doxorubicin without induction of drug resistance. Human Wharton's jelly derived mesenchymal stem cells exerted cytotoxic effect on leukemia cells. Addition of that effect to anticancer effect of chemotherapeutic agents can leads to cytotoxic drug dose reduction and diminished side effects.
间充质干细胞（MSC）疗法正逐步走向临床应用。近期证据证实了间充质干细胞的抗癌作用。然而，包括癌症类型、MSC来源、研究设计和动物模型等多种因素会对最终结果产生影响。沃顿胶——一种新获批的MSC来源——相较于传统来源的骨髓具有优势；因此，对其医学效果进行研究可能会产生有益成果。在本研究中，我们检测了人脐带沃顿胶MSC分泌组对K562人白血病细胞的细胞毒性和促凋亡作用。通过组织块培养法从人脐带沃顿胶中分离出间充质干细胞，然后根据国际细胞治疗协会（ISCT）标准（形态学和贴壁生长特性、表面抗原性及分化潜能）进行鉴定。收集MSC分泌组，并使用BrdU细胞增殖试验和膜联蛋白V-碘化丙啶（Annexin V-PI）染色评估其与阿霉素联合作用时对K562细胞的细胞毒性和促凋亡作用。我们的结果表明，间充质干细胞分泌组对K562癌细胞具有抗增殖作用，该作用还增强了阿霉素的细胞毒性且未诱导耐药性。人脐带沃顿胶来源的间充质干细胞对白血病细胞具有细胞毒性作用。将该作用与化疗药物的抗癌作用相加，可降低细胞毒性药物剂量并减少副作用。
