α-硫辛酸对腹腔粘连的影响：大鼠模型的实验研究

Effects of alpha lipoic acid on intra-abdominal adhesion: an experimental study in a rat model.

作者信息

Diken Allahverdi Tülay, Allahverdi Ertuğrul, Yayla Sadık, Deprem Turgay, Merhan Oguz, Vural Sevil, Sülü Barlas, Günerhan Yavuz, Köksal Neşet

机构信息

Department of General Surgery, Kafkas University Faculty of Medicine, Kars, Turkey.

Department of Orthopedic and Traumatology, Kars State Hospital, Kars, Turkey.

出版信息

Ulus Travma Acil Cerrahi Derg. 2015 Jan;21(1):9-14. doi: 10.5505/tjtes.2015.15985.

DOI:10.5505/tjtes.2015.15985

PMID:25779706

Abstract

BACKGROUND

This study was performed to determine the effect of alpha lipoic acid, a powerful antioxidant, on intra-abdominal adhesion formation.

METHODS

Twenty-four female Wistar Albino rats weighing 250-300 g were used in this study conducted on three groups consisting of the alpha lipoic acid group (Group I, n=8), control group (Group II, n=8), and sham group (Group III, n=8). After performing laparotomy with a midline incision under general anesthesia, the adhesion model was created on the antimesenteric side of the caecum in Group I and Group II. 50 mg/kg alpha-lipoic acid was administered intraperitoneally (IP) in Group I while the surgical procedure was performed but no drugs administered in Group II. Only laparotomy was performed in Group III. Rats were sacrificed at the end of the tenth day.

RESULTS

Macroscopic scoring was performed, tissue samples were obtained and subjected to biochemical and histopathological evaluation. The degree of adhesion and malondialdehyde level decreased (p<0.01), and glutathione levels had increased (p<0.01) in Group I compared to Group II in macroscopic scoring.

CONCLUSION

Alpha lipoic acid was found to significantly decrease (p<0.01) intra-abdominal adhesion when administered IP compared to the control group.

摘要

背景

本研究旨在确定一种强大的抗氧化剂α硫辛酸对腹腔粘连形成的影响。

方法

本研究使用24只体重250 - 300克的雌性Wistar白化大鼠，分为三组，即α硫辛酸组（第一组，n = 8）、对照组（第二组，n = 8）和假手术组（第三组，n = 8）。在全身麻醉下经中线切口进行剖腹手术后，在第一组和第二组的盲肠系膜对侧建立粘连模型。第一组在手术过程中腹腔内注射（IP）50毫克/千克α硫辛酸，而第二组只进行手术操作未给药。第三组仅进行剖腹手术。在第十天结束时处死大鼠。