Subochev Pavel, Orlova Anna, Shirmanova Marina, Postnikova Anna, Turchin Ilya
Institute of Applied Physics RAS, 46 Ulyanov Street, Nizhniy Novgorod, Russia.
Lobachevsky State University of Nizhny Novgorod, 19 Gagarin Avenue, Nizhniy Novgorod, Russia ; Nizhny Novgorod State Medical Academy, Nizhniy Novgorod, Russia.
Biomed Opt Express. 2015 Jan 29;6(2):631-8. doi: 10.1364/BOE.6.000631. eCollection 2015 Feb 1.
We propose the use of thermoelastic (TE) excitation of an ultrasonic (US) detector by backscattered laser radiation as a means of upgrading a single-modality photoacoustic (PA) microscope to dual-modality PA/US imaging at minimal cost. The upgraded scanning head of our dual-modality microscope consists of a fiber bundle with 14 output arms and a 32MHz polyvinylidene difluoride (PVDF) detector with a 34 MHz bandwidth (-6 dB level), 12.7 mm focal length, and a 0.25 numerical aperture. A single optical pulse delivered through the fiber bundle to the biotissue being investigated, in combination with a metalized surface on the PVDF detector allows us to obtain both PA and US A-scans. To demonstrate the in vivo capabilities of the proposed method we present the results of bimodal imaging of the brain of a newborn rat, a mouse tail and a mouse tumor.
我们提议利用背向散射激光辐射对超声（US）探测器进行热弹性（TE）激发，作为以最低成本将单模态光声（PA）显微镜升级为双模态PA/US成像的一种手段。我们双模态显微镜升级后的扫描头由一个具有14个输出臂的光纤束和一个32MHz的聚偏二氟乙烯（PVDF）探测器组成，该探测器具有34MHz带宽（-6dB电平）、12.7mm焦距和0.25的数值孔径。通过光纤束向正在研究的生物组织发射单个光脉冲，结合PVDF探测器上的金属化表面，使我们能够获得PA和US A扫描。为了证明所提方法的体内成像能力，我们展示了新生大鼠大脑、小鼠尾巴和小鼠肿瘤的双模态成像结果。
