富硒茶多糖的提取、表征及抗氧化活性研究。
Extraction, characterization and antioxidant activities of Se-enriched tea polysaccharides.
机构信息
State Key Laboratory of Dairy Biotechnology, Bright Dairy Co., Ltd, 1518 West Jiangchang Rd, Shanghai 200436, PR China; Institute of Food Engineering, College of Life & Environment Science, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, PR China.
Institute of Food Engineering, College of Life & Environment Science, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, PR China.
出版信息
Int J Biol Macromol. 2015;77:76-84. doi: 10.1016/j.ijbiomac.2015.02.052. Epub 2015 Mar 14.
Se-polysaccharides from Se-enriched tea leaves were purified by DEAE-sepharose fast flow gel column (2.5×60cm) and three polysaccharide fractions (Se-TPS1, Se-TPS2, and Se-TPS3) were isolated and purified with yields of 6.5, 37.14, and 8.57%, respectively. The average sizes of Se-TPS1 and Se-TPS2 were determined by HPGPC system, with molecular weights of 1.1×10(5) and 2.4×10(5)Da, respectively. Se-TPS3 was a polysaccharide polymer with two peaks with molecular weights of 9.2×10(5) and 2.5×10(5)Da. Monosaccharide components analysis by ion chromatography revealed Se-polysaccharides were acidic polysaccharoses and different from each other in monosaccharide kinds and molar ratio. Elements of Se, C, H, N, S, and 14 kinds of mineral elements were analyzed by AFS, EA, and ICP-AES, respectively. Spectral analysis (IR and UV) indicated Se-polysaccharides were typical glycoproteins. Morphological analyses of the samples were determined by SEM and AFM. In addition, the DPPH and superoxide radicals scavenging activities were also discussed to assess antioxidant activities of the samples, and Se-polysaccharides showed higher antioxidant activities compared to the ordinary polysaccharides.
富硒茶中的 Se 多糖通过 DEAE-琼脂糖 fast flow 凝胶柱（2.5×60cm）进行纯化，分离并纯化出三种多糖级分（Se-TPS1、Se-TPS2 和 Se-TPS3），产率分别为 6.5%、37.14%和 8.57%。通过 HPGPC 系统测定 Se-TPS1 和 Se-TPS2 的平均分子量分别为 1.1×10(5)和 2.4×10(5)Da。Se-TPS3 是一种多糖聚合物，有两个峰，分子量分别为 9.2×10(5)和 2.5×10(5)Da。离子色谱法的单糖成分分析表明，Se 多糖是酸性多糖，并且在单糖种类和摩尔比方面彼此不同。AFS、EA 和 ICP-AES 分别分析了 Se、C、H、N、S 和 14 种矿物质元素。光谱分析（IR 和 UV）表明 Se 多糖是典型的糖蛋白。通过 SEM 和 AFM 对样品的形态进行了分析。此外，还讨论了 DPPH 和超氧自由基清除活性，以评估样品的抗氧化活性，与普通多糖相比，Se 多糖具有更高的抗氧化活性。
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