School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223001, China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaian 223300, China; Huaian High-Tech Research Institute of Nanjing University, Huaian 223001, China.
Carbohydr Polym. 2014 Jan 30;101:379-85. doi: 10.1016/j.carbpol.2013.09.031. Epub 2013 Sep 23.
Four methods for extracting polysaccharides from Boletus edulis, namely, hot-water extraction, ultrasonic clearer extraction, static probe ultrasonic extraction, and pulsed counter-current probe ultrasonic extraction (CCPUE), were studied. Results showed that CCPUE has the highest extraction efficiency among the methods studied. Under optimal CCPUE conditions, a B. edulis polysaccharide (BEP) yield of 8.21% was obtained. Three purified fractions, BEP-I, BEP-II, and BEP-III, were obtained through sequential purification by DEAE-52 and Sephadex G-75 chromatography. The average molecular weights of BEP-I, BEP-II, and BEP-III were 10,278, 23,761, and 42,736 Da, respectively. The polysaccharides were mainly composed of xylose, mannose, galactose, and glucose; of these, mannose contents were the highest. The antioxidant activities of the BEPs were further investigated by measurement of their ability to scavenge DPPH and hydroxyl radicals as well as their reducing power. The results indicated that the BEPs have good antioxidant activity.
研究了从牛肝菌中提取多糖的四种方法，即热水提取法、超声提取法、静态探针超声提取法和脉冲逆流探针超声提取法（CCPUE）。结果表明，CCPUE 在研究的方法中具有最高的提取效率。在最优的 CCPUE 条件下，得到了 8.21%的牛肝菌多糖（BEP）产量。通过 DEAE-52 和 Sephadex G-75 色谱的顺序纯化，得到了三个纯化级分 BEP-I、BEP-II 和 BEP-III。BEP-I、BEP-II 和 BEP-III 的平均分子量分别为 10278、23761 和 42736 Da。多糖主要由木糖、甘露糖、半乳糖和葡萄糖组成，其中甘露糖含量最高。通过测定 DPPH 和羟基自由基清除能力以及还原能力进一步研究了 BEP 的抗氧化活性。结果表明，BEP 具有良好的抗氧化活性。
