College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, 310018, China.
Institute of Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, China.
Plant Foods Hum Nutr. 2024 Mar;79(1):173-181. doi: 10.1007/s11130-024-01145-2. Epub 2024 Jan 25.
The aim of this study was to investigate the functional mechanism of Wuniuzao dark tea polysaccharide (WDTP) that protect against hyperlipidemia in mice induced by high-fat diet. WDTP was extracted by hot water, isolated and purified by DEAE-52 chromatography and characterized by high-performance liquid chromatograph (HPLC), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). Different doses (200 or 800 mg/kg/day) of WDTP were orally administered to mice induced by high-fat diet to evaluate the mechanism of WDTP regulating lipid metabolism. And these results showed that average molecular weight of WDTP was nearly 63,869 Da. And WDTP intervention significantly reduced body weight, lipid accumulation, and modulated blood lipid levels. The mechanism of WDTP ameliorating lipid metabolism was associated with regulating the expression of lipid metabolism-related genes and serum exosomes miR-19b-3p, and modulating the community structure of gut microbiota in mice.
本研究旨在探讨五牛早黑茶多糖（WDTP）对高脂饮食诱导的小鼠高血脂的作用机制。WDTP 采用热水提取，DEAE-52 层析柱分离纯化，高效液相色谱（HPLC）、傅里叶变换红外光谱（FT-IR）和扫描电子显微镜（SEM）进行表征。WDTP 以不同剂量（200 或 800mg/kg/天）灌胃高脂饮食诱导的小鼠，评价 WDTP 调节脂代谢的作用机制。结果表明，WDTP 的平均分子量接近 63869Da。WDTP 干预可显著降低体重、脂质蓄积，调节血脂水平。WDTP 改善脂代谢的机制与调节脂代谢相关基因和血清外泌体 miR-19b-3p 的表达以及调节小鼠肠道微生物群的群落结构有关。
