Baggiolini M, Horisberger U, Gennaro R, Dewald B
Lab Invest. 1985 Feb;52(2):151-8.
The production of three populations of granules during maturation and their occurrence in the neutrophils of a number of ruminants is described. Bone marrow samples from cows, sheep, and goats were fixed, reacted for peroxidase, and examined by transmission electron microscopy. Granule formation was found to occur at three subsequent stages. As in other species including man, it begins with the production of peroxidase-positive granules and is concluded with the production of moderately dark-staining, peroxidase-negative granules of similar size. These two organelles have the characteristics of the azurophil or primary and specific or secondary granules of other species. At an intermediate stage, a different type of peroxidase-negative granule, the large granule, is formed. Its profile is round and markedly larger than those of azurophil and specific granules. The large granules initially have a loose, undulated membrane and fill gradually with matrix which in early forms consists of a central core. Fully developed large granules have a sharply defined membrane and a pale, very uniform content. The two types of peroxidase-negative granules can be distinguished by their sizes and times of formation and also by the appearance of their content which is more coarse and darker in the specific granules. Mature neutrophils from cows, goats, sheep, and an ibex were also examined. Two features are common to all cells viewed: the presence of three types of granules and the preponderance of the large granule population. The results of this study, together with evidence from cell fractionation experiments, suggest that the large granules are a characteristic property of the neutrophils of ruminants.
本文描述了反刍动物中性粒细胞成熟过程中三种颗粒群的产生及其情况。采集牛、羊和山羊的骨髓样本，进行固定、过氧化物酶反应，然后用透射电子显微镜检查。发现颗粒形成发生在三个连续阶段。与包括人类在内的其他物种一样，颗粒形成始于过氧化物酶阳性颗粒的产生，结束于大小相似、中等深染、过氧化物酶阴性颗粒的产生。这两种细胞器具有其他物种嗜天青颗粒或初级颗粒以及特异性颗粒或次级颗粒的特征。在中间阶段，形成了一种不同类型的过氧化物酶阴性颗粒，即大颗粒。其形态呈圆形，明显大于嗜天青颗粒和特异性颗粒。大颗粒最初有一层松散、起伏的膜，逐渐充满基质，早期基质由一个中央核心组成。完全成熟的大颗粒有清晰界定的膜和浅色、非常均匀的内容物。两种过氧化物酶阴性颗粒可通过其大小、形成时间以及内容物外观来区分，特异性颗粒的内容物更粗糙、颜色更深。还对牛、山羊、绵羊和一只野生山羊的成熟中性粒细胞进行了检查。所有观察到的细胞都有两个共同特征：存在三种类型的颗粒，且大颗粒群占优势。本研究结果与细胞分级分离实验的证据表明，大颗粒是反刍动物中性粒细胞的一个特征属性。
