Pichler Renate, Borena Wegene, Schäfer Georg, Manzl Claudia, Culig Zoran, List Sebastian, Neururer Sabrina, Von Laer Dorothee, Heidegger Isabel, Klocker Helmut, Horninger Wolfgang, Steiner Hannes, Brunner Andrea
Division of Experimental Urology, Department of Urology, Medical University of Innsbruck, Innsbruck, Austria.
Section of Virology, Department of Hygiene, Microbiology and Social Medicine, Innsbruck, Austria.
World J Urol. 2015 Dec;33(12):2145-51. doi: 10.1007/s00345-015-1539-y. Epub 2015 Mar 19.
To clarify the role of human papillomavirus (HPV) in non-muscle invasive bladder cancer, HPV-DNA was scrutinized in formalin-fixed, paraffin-embedded (FFPE) bladder cancer tissue using single-step PCR (HPV L1) for HPV detection, followed by reverse line blot (RLB) for genotyping.
A total of 186 patients who underwent transurethral resection of the bladder due to primary, non-muscle invasive bladder cancer from 2006 to 2009 were reviewed. A positive control group of 22 cervical tissues with cervical carcinoma was included.
Histology confirmed urothelial carcinoma in all patients: primary CIS, pTa, pT1 and pTa + pT1 in 14 (7.5 %), 134 (72 %), 36 (19.4 %) and two (1.1 %) patients, respectively. A total of 119 (63.9 %) of them were classified as low-risk, while 67 (36.1 %) were high-risk cancers. Tumor recurrence and progression (≥pT2) were seen in 79 and 11 patients (mean follow-up 45 months). The presence of HPV-DNA by single-step PCR was detected in four (2.2 %) patients. HPV 16 and HPV 6 were positive in two (1.1 %) and one (0.6 %) patient, respectively In one case, no HPV genotype listed on the RLB assay could be identified. In the control group, the HPV infection rate was 100 %: HPV 16 in 12 (54.6 %) patients, HPV 16/18 in four (18.3 %) patients, HPV 18 in two (9.1 %) patients, HPV 16/45 in one patient (4.5 %), HPV 18/33 in one (4.5 %) patient, HPV 16/33 in one (4.5 %) patient and HPV 33 in one (4.5 %) patient.
Our study demonstrates low prevalence of HPV infection in FFPE bladder cancer tissue, arguing against the etiological role of HPV in non-muscle urothelial carcinogenesis.
为阐明人乳头瘤病毒（HPV）在非肌层浸润性膀胱癌中的作用，采用单步聚合酶链反应（HPV L1）检测福尔马林固定、石蜡包埋（FFPE）膀胱癌组织中的HPV-DNA，随后进行反向线印迹（RLB）基因分型。
回顾性分析2006年至2009年因原发性非肌层浸润性膀胱癌接受经尿道膀胱切除术的186例患者。纳入22例宫颈癌宫颈组织作为阳性对照组。
所有患者组织学均确诊为尿路上皮癌：分别有14例（7.5%）为原发性原位癌（CIS）、134例（72%）为pTa期、36例（19.4%）为pT1期、2例（1.1%）为pTa + pT1期。其中共有119例（63.9%）为低危癌，67例（36.1%）为高危癌。79例和11例患者出现肿瘤复发和进展（≥pT2期）（平均随访45个月）。单步聚合酶链反应检测发现4例（2.2%）患者存在HPV-DNA。HPV 16和HPV 6分别在2例（1.1%）和1例（0.6%）患者中呈阳性。1例患者无法鉴定出RLB检测中列出的HPV基因型。对照组中，HPV感染率为100%：12例（54.6%）患者为HPV 16，4例（18.3%）患者为HPV 16/18，2例（9.1%）患者为HPV 18，1例患者（4.5%）为HPV 16/45，1例（4.5%）患者为HPV 18/33，1例（4.5%）患者为HPV 16/33，1例（4.5%）患者为HPV 33。
我们的研究表明FFPE膀胱癌组织中HPV感染率较低，这与HPV在非肌层尿路上皮癌发生中的病因学作用相悖。
