Zhu Sidong, Lu Yanna, Xu Xu, Chen Jigang, Yang Jifang, Ma Xiangdong
College of Biological and Environmental Sciences, Zhejiang Wanli University, No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of China.
Folia Microbiol (Praha). 2015 Jul;60(4):309-16. doi: 10.1007/s12223-015-0386-y. Epub 2015 Mar 19.
We describe the isolation and identification of a gene encoding 4-hydroxyphenylpyruvate dioxygenase (HPPD (EC 1.13.11.27)) from the red-brown pigment-producing bacterium Alteromonas stellipolaris LMG 21856. HPPD directs the synthesis of 2,5-dihydroxyphenylacetic acid (homogentisic acid (HGA)). The sequence of the deduced peptide showed homology to HPPDs from other strains of Alteromonas; the greatest similarity was to the hypothetical HPPD from Alteromonas sp. SN2. As observed for HPPDs from other sources, expression of the A. stellipolaris HPPD gene in Escherichia coli cells could be detected by the gradual development of a brown pigment in cultures as a result of the spontaneous oxidation and polymerization of HGA.
我们描述了从产红棕色色素的细菌星极交替单胞菌LMG 21856中分离和鉴定编码4-羟基苯丙酮酸双加氧酶（HPPD，EC 1.13.11.27）的基因。HPPD指导2,5-二羟基苯乙酸（尿黑酸（HGA））的合成。推导肽的序列与来自其他交替单胞菌菌株的HPPD具有同源性；与来自交替单胞菌属SN2的假定HPPD最为相似。正如在其他来源的HPPD中观察到的那样，由于HGA的自发氧化和聚合，培养物中棕色色素的逐渐形成可检测到星极交替单胞菌HPPD基因在大肠杆菌细胞中的表达。
