Department of Chemistry University of Sheffield, Sheffield, S3 7HF, UK.
Dalton Trans. 2017 May 9;46(18):6079-6086. doi: 10.1039/c7dt00801e.
Herein we describe the DNA binding properties of two new water-soluble ruthenium complexes; experimental and computational data reveal that both complexes display dual emission from MLCT and LLCT excited states. The interaction of the new complexes with DNA was also investigated. Although one of the complexes only binds DNA though groove binding, the second complex has separate ligands capable of groove binding and intercalation. Nevertheless, it was found that both complexes interact with duplex DNA with high affinity. DNA induced distinctive changes in the emission of the complexes; although the groove binding complex only displays a modest increase in emission on binding, the complex that contains the intercalating Ru(dppz) moiety displays a large increase in MLCT-based emission on DNA binding while emission from the LLCT excited state is unaffected. This means that the complex functions as the first ratiometric sensor for DNA.
在这里，我们描述了两个新的水溶性钌配合物的 DNA 结合特性；实验和计算数据表明，这两个配合物都显示了来自 MLCT 和 LLCT 激发态的双重发射。还研究了新配合物与 DNA 的相互作用。尽管其中一个配合物仅通过沟结合与 DNA 结合，第二个配合物具有能够进行沟结合和嵌入的单独配体。然而，发现这两个配合物都与双链 DNA 具有高亲和力相互作用。DNA 诱导了配合物发射的明显变化；虽然沟结合配合物在结合时仅显示出发射的适度增加，但含有嵌入 Ru(dppz)部分的配合物在 DNA 结合时显示出基于 MLCT 的发射的大幅增加，而 LLCT 激发态的发射不受影响。这意味着该配合物是第一个用于 DNA 的比率型传感器。
