Roustan C, Benyamin Y, Boyer M, Bertrand R, Audemard E, Jauregui-Adell J
FEBS Lett. 1985 Feb 11;181(1):119-23. doi: 10.1016/0014-5793(85)81125-x.
The effect of Mg2+ ions on the conformation of G-actin and in particular on the accessibility of its antigenic regions has been tested. Experiments were performed with G-actin coupled to Sepharose 4B which was, therefore, maintained in the monomeric state. The results presented her show that the 2mM MgCl2-perturbed antigenic site is located in a central region of the actin sequence.
已测试了Mg2+离子对G-肌动蛋白构象的影响,特别是对其抗原区域可及性的影响。实验是用偶联到琼脂糖4B上的G-肌动蛋白进行的,因此其保持在单体状态。此处给出的结果表明,2mM MgCl2干扰的抗原位点位于肌动蛋白序列的中央区域。