Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
Department of Chemistry, University of Wisconsin-Platteville, 1 University Plaza, Platteville, WI 53818-3099, USA.
Biosens Bioelectron. 2018 Jan 15;99:34-39. doi: 10.1016/j.bios.2017.07.037. Epub 2017 Jul 14.
A molecularly imprinted electrochemical sensor for neuron specific enolase (NSE) was developed by electrochemical polymerizing ionic liquid, which was functionalized with pyrrole moiety, in between gold nanoarrays. A well-defined 3D structured gold nanoarray was fabricated on a glassy carbon electrode (GCE) surface by using template-assisted electrochemical deposition technique. 1-(3-mercaptopropyl)-3-vinyl-imidazolium tetrafluoroborate was self-assembled onto the surface of gold nanoarrays to produce active sites for anchoring the molecularly imprinted film. Subsequently, an electrochemical polymerization procedure was carried out in an aqueous solution containing 1,3-di(3-N-pyrrolpropyl)imidazolium bromine ionic liquid and neuron specific enolase (NSE). After removing NSE templates, a molecularly imprinted sensor was successfully fabricated. The sensor showed high selectivity and sensitivity towards NSE, produced a linear response in the concentration range from 0.01 to 1.0ngmL and had a detection limit of 2.6pgmL with an incubation time of 15min. The developed sensor was demonstrated successful in determining NSE in clinical serum samples.
一种基于分子印迹电化学传感器的神经元特异性烯醇化酶（NSE）被开发出来，该传感器是通过在金纳米阵列之间电化学聚合功能化吡咯部分的离子液体而制备的。通过使用模板辅助电化学沉积技术，在玻璃碳电极（GCE）表面上制备了具有良好定义的 3D 结构的金纳米阵列。1-（3-巯丙基）-3-乙烯基-咪唑四氟硼酸盐自组装到金纳米阵列的表面上，产生用于固定分子印迹膜的活性位点。随后，在含有 1,3-二（3-N-吡咯丙基）咪唑溴离子液体和神经元特异性烯醇化酶（NSE）的水溶液中进行了电化学聚合过程。在去除 NSE 模板后，成功制备了分子印迹传感器。该传感器对 NSE 表现出高选择性和灵敏度，在 0.01 至 1.0ngmL 的浓度范围内产生线性响应，检测限为 2.6pgmL，孵育时间为 15min。该开发的传感器成功地用于测定临床血清样本中的 NSE。
