Srikanta S, Rabizadeh A, Omar M A, Eisenbarth G S
Diabetes. 1985 Mar;34(3):300-5. doi: 10.2337/diab.34.3.300.
Assays for islet cell antibodies (ICA) are finding increasing application in clinical diabetology. We have developed a new islet cell antibody assay system (ICA-pA), whose salient features include: (1) utilization of fluorescein-conjugated staphylococcal protein A as a standard second-step reagent, the advantages of this approach being improved "signal" (islet)/"noise" (acini) ratio due to reduction of interfering background acinar pancreatic staining, and facilitation of assay standardization provided by the use of a chemically pure conjugated protein A reagent; (2) monoclonal antibody counterstaining with rhodamine-conjugated BISL-32 for the rapid identification of islets in pancreatic sections; and (3) quantitation of circulating serum ICA by microimmunofluorometric techniques.
胰岛细胞抗体（ICA）检测在临床糖尿病学中的应用越来越广泛。我们开发了一种新的胰岛细胞抗体检测系统（ICA-pA），其显著特点包括：（1）使用荧光素偶联的葡萄球菌蛋白A作为标准的第二步试剂，这种方法的优点是由于减少了干扰性的腺泡胰腺背景染色，提高了“信号”（胰岛）/“噪声”（腺泡）比值，并通过使用化学纯的偶联蛋白A试剂促进了检测标准化；（2）用罗丹明偶联的BISL-32进行单克隆抗体复染，以快速识别胰腺切片中的胰岛；（3）通过微量免疫荧光技术对循环血清ICA进行定量。
