Cefalù S, Lena A M, Vojtesek B, Musarò A, Rossi A, Melino G, Candi E
a Istututo Dermopatico dell'Immacolata ; IDI-IRCCS ; Rome , Italy.
Cell Cycle. 2015;14(6):894-901. doi: 10.4161/15384101.2014.988021.
p53 family members, p63 and p73, play a role in controlling early stage of myogenic differentiation. We demonstrated that TAp63gamma, unlike the other p53 family members, is markedly up-regulated during myogenic differentiation in murine C2C7 cell line. We also found that myotubes formation was inhibited upon TAp63gamma knock-down, as also indicated by atrophyic myotubes and reduction of myoblasts fusion index. Analysis of TAp63gamma-dependend transcripts identified several target genes involved in skeletal muscle contractility energy metabolism, myogenesis and skeletal muscle autocrine signaling. These results indicate that TAp63gamma is a late marker of myogenic differentiation and, by controlling different sub-sets of target genes, it possibly contributes to muscle growth, remodeling, functional differentiation and tissue homeostasis.
p53家族成员p63和p73在控制成肌分化的早期阶段发挥作用。我们证明，与其他p53家族成员不同，TAp63γ在小鼠C2C7细胞系的成肌分化过程中显著上调。我们还发现，敲低TAp63γ会抑制肌管形成，萎缩的肌管和成肌细胞融合指数降低也表明了这一点。对TAp63γ依赖性转录本的分析确定了几个参与骨骼肌收缩能量代谢、肌生成和骨骼肌自分泌信号传导的靶基因。这些结果表明，TAp63γ是成肌分化的晚期标志物，通过控制不同的靶基因子集，它可能有助于肌肉生长、重塑、功能分化和组织稳态。
