Turk J Med Sci. 2015;45(1):60-9. doi: 10.3906/sag-1311-102.
BACKGROUND/AIM: β-Lactamase production is considered one of the most important resistance mechanisms amongvirulent Pseudomonas aeruginosa isolates. The aim of this study was to compare the production and antimicrobial resistance patterns of some virulence factors in extended spectrum β-lactamase (ESβL)-producing and nonproducing P. aeruginosa clinical isolates.
Out of 183 different clinical specimens, 104 Pseudomonas aeruginosa isolates were recovered. The isolates were screened for ESβL production using the double disk diffusion test and phenotypic confirmatory disk diffusion test. All isolates were tested for susceptibility to 25 antimicrobials, as well as for expression of various virulence factors including pigment, hemolysin, gelatinase, protease, lipase, rhamnolipids, biofilm, and cell surface hydrophobicity. The results of ESβL producers and honproducers were statistically compared.
All isolates showed a high frequency of multiple resistance to at least 14 and up to 25 of the tested antimicrobials. Nevertheless, most virulence factors were produced at higher rates in ESβL-producing than in ESβL-nonproducing Pseudomonas aeruginosa isolates.
The results of this study suggest a correlation between ESβL phenotype and the production of some factors that are reported to be involved in the virulence of P. aeruginosa.
背景/目的：产β-内酰胺酶被认为是铜绿假单胞菌毒力株中最重要的耐药机制之一。本研究旨在比较产和不产超广谱β-内酰胺酶（ESβL）的铜绿假单胞菌临床分离株中某些毒力因子的产生和抗菌药物耐药模式。
从 183 份不同的临床标本中，共分离出 104 株铜绿假单胞菌。采用双纸片扩散试验和表型确证纸片扩散试验筛选 ESβL 产生菌。所有分离株均进行 25 种抗菌药物的药敏试验，以及各种毒力因子（包括色素、溶血素、明胶酶、蛋白酶、脂肪酶、鼠李糖脂、生物膜和细胞表面疏水性）的表达检测。比较 ESβL 产生菌和非产生菌的结果。
所有分离株对至少 14 种和最多 25 种测试抗菌药物均表现出高频度的多重耐药性。然而，产 ESβL 的铜绿假单胞菌分离株比非产 ESβL 的分离株产生更多的毒力因子。
本研究结果提示 ESβL 表型与某些被报道与铜绿假单胞菌毒力有关的因子的产生之间存在相关性。
