2000-2010 年土耳其临床和环境样本中肠炎沙门氏菌的多州暴发的分子流行病学调查。
A molecular epidemiological investigation of multistate outbreaks of Salmonella Enteritidis from clinical and environmental samples in Turkey, 2000-2010.
出版信息
Turk J Med Sci. 2015;45(1):76-83. doi: 10.3906/sag-1401-33.
BACKGROUND/AIM: To analyze interrelated Salmonella Enteritidis strains isolated in Turkey. Fifty-five S. Enteritidis surveillance strains were isolated from human feces and environmental samples from different regions in Turkey between 2000 and 2010.
MATERIALS AND METHODS
Clinical isolates were selected from different outbreaks in the Turkish National Reference Laboratory. All isolates were submitted to antimicrobial susceptibility test, plasmid profile analysis, and XbaI-digested pulsed-field gel electrophoresis.
RESULTS
The strains were scanned against 20 antibiotics and for 3 of them (amikacin, ciprofloxacin, gentamicin), all strains were found to be sensitive. Five isolates had no plasmid. Most of test strains carried the 57-kb plasmid in common and 15 genotypes were identified among the 55 isolates. Six genotypes were related closely, 3 genotypes were undistinguished, and 6 genotypes were unrelated.
CONCLUSION
To our knowledge, this is the first report on the phenotypic and molecular characterization of S. Enteritidis isolates from both environmental samples and clinical isolates in Turkey.
背景/目的：分析在土耳其分离的相关肠炎沙门氏菌菌株。2000 年至 2010 年间，从土耳其不同地区的人类粪便和环境样本中分离出 55 株肠炎沙门氏菌监测菌株。
材料和方法
从土耳其国家参考实验室的不同暴发中选择临床分离株。所有分离株均进行了药敏试验、质粒图谱分析和 XbaI 消化脉冲场凝胶电泳。
结果
这些菌株被扫描了 20 种抗生素，其中 3 种（阿米卡星、环丙沙星、庆大霉素）所有菌株均敏感。5 株分离株无质粒。大多数试验菌株携带共同的 57-kb 质粒，在 55 株分离株中鉴定出 15 种基因型。6 种基因型密切相关，3 种基因型无差异，6 种基因型无关。
结论
据我们所知，这是首次报道来自土耳其环境样本和临床分离株的肠炎沙门氏菌的表型和分子特征。
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