Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Food Microbiology Research Centre, Tehran University of Medical Sciences, Tehran, Iran.
J Glob Antimicrob Resist. 2018 Mar;12:220-226. doi: 10.1016/j.jgar.2017.10.005. Epub 2017 Oct 16.
Salmonella enterica serotype Enteritidis is a major serotype associated with human salmonellosis. The main objective of this study was to determine the antibiotic susceptibility patterns and the presence of virulence-associated genes among S. Enteritidis strains isolated from patients with gastroenteritis in Tehran, Iran.
Over a period of 14 months (May 2015 to July 2016), 44 S. Enteritidis isolates recovered from clinical sources were characterised for antimicrobial susceptibility and virulence genes. Possible genetic relatedness among the strains was also assessed using pulsed-field gel electrophoresis (PFGE).
Salmonella Enteritidis isolates showed high rates of resistance to ciprofloxacin (90.9%) and nalidixic acid (77.3%). Of the 44 S. Enteritidis isolates, 30 (68.2%) were resistant to three or more antibiotics. Twenty-two different antimicrobial resistance patterns were detected among the isolates. The most frequent resistance type was antibiotype 14 (resistance to ciprofloxacin, cefuroxime and nalidixic acid), occurring in 8 (18.2%) of the isolates. Notably, all of the isolates carried invA, sefA, sipA and sopE2 virulence genes. Furthermore, 17 virulence profiles were observed among the strains. The most common virulence profile was VP1 (n=17; 38.6%), harbouring all of the virulence genes. Two distinct PFGE patterns were observed among 44S. Enteritidis isolates. There was no association between virulence profiles or antibiotypes and PFGE clusters.
Overall, this study provides valuable information on the virulence gene content, antibiotic resistance and genetic diversity of S. Enteritidis isolated from human sources in Iran.
肠炎沙门氏菌血清型肠炎亚种是与人类沙门氏菌病相关的主要血清型。本研究的主要目的是确定从伊朗德黑兰胃肠炎患者中分离的肠炎沙门氏菌菌株的抗生素药敏模式和毒力相关基因的存在情况。
在 14 个月的时间内（2015 年 5 月至 2016 年 7 月），从临床来源中分离出 44 株肠炎沙门氏菌，对其进行了抗生素药敏性和毒力基因的鉴定。还使用脉冲场凝胶电泳（PFGE）评估了菌株之间的可能遗传相关性。
肠炎沙门氏菌分离株对环丙沙星（90.9%）和萘啶酸（77.3%）的耐药率较高。在 44 株肠炎沙门氏菌分离株中，有 30 株（68.2%）对三种或三种以上抗生素耐药。在分离株中检测到 22 种不同的抗生素耐药模式。最常见的耐药类型是抗生素型 14（对环丙沙星、头孢呋辛和萘啶酸耐药），在 8 株（18.2%）分离株中出现。值得注意的是，所有分离株均携带 invA、sefA、sipA 和 sopE2 毒力基因。此外，在菌株中观察到 17 种毒力谱。最常见的毒力谱是 VP1（n=17；38.6%），携带所有毒力基因。在 44 株肠炎沙门氏菌分离株中观察到两种不同的 PFGE 模式。毒力谱或抗生素型与 PFGE 簇之间没有关联。
总体而言，本研究提供了有关从伊朗人类来源分离的肠炎沙门氏菌的毒力基因含量、抗生素耐药性和遗传多样性的有价值信息。
