Turk J Med Sci. 2015;45(1):105-11. doi: 10.3906/sag-1402-24.
BACKGROUND/AIM: Since blood bags have the ability for diffusion of gases, we investigated whether hyperbaric oxygen (HBO) exposure affects several vital parameters of stored blood.
Bloods obtained from the same persons were used as both control and HBO groups and stored in pediatric bags with citrate-phosphate-dextrose solution. HBO administration was performed at 2.5 atm for 90 min, started 1 day after blood collection and repeated every 2 days for a total of 10 times. The study was terminated on the 21st day. Complete blood count, glucose, pH, and osmotic fragility values were measured every week.
Glucose and pH levels decreased in stored blood. In the HBO-exposed group, these decreases were less than in the control. In addition, mean corpuscular and platelet volumes tended to increase during storing process, but with HBO, these indexes remained lower, near physiologic levels. Another interesting finding of the study was the relative stable osmotic fragility ratio in the HBO group compared to the control blood.
HBO exposure has positive effects on pH, stability of erythrocytes, and energy source (glucose) of the medium. Thus, we concluded that HBO may be a useful application for life and quality of stored blood.
背景/目的：由于血袋具有气体扩散的能力，我们研究了高压氧（HBO）暴露是否会影响储存血液的几个重要参数。
从同一供体采集的血液分别作为对照和 HBO 组，用含有柠檬酸盐-磷酸盐-葡萄糖溶液的小儿血袋储存。HBO 治疗于采血后第 1 天开始，以 2.5 个大气压暴露 90 分钟，每 2 天重复 1 次，共 10 次。研究于第 21 天结束。每周测量全血细胞计数、葡萄糖、pH 值和渗透脆性值。
储存血液中的葡萄糖和 pH 值降低。在 HBO 暴露组中，这些降低的程度低于对照组。此外，在储存过程中，平均红细胞体积和血小板体积趋于增加，但 HBO 组这些指标仍较低，接近生理水平。该研究的另一个有趣发现是，与对照组血液相比，HBO 组的渗透脆性比值相对稳定。
HBO 暴露对 pH 值、红细胞稳定性和介质中的能量来源（葡萄糖）有积极影响。因此，我们得出结论，HBO 可能是储存血液的生命和质量的有用应用。
