大肠杆菌素E1分子功能组织的遗传学研究。
Genetic study of the functional organization of the colicin E1 molecule.
作者信息
Suit J L, Fan M L, Kayalar C, Luria S E
出版信息
J Bacteriol. 1985 Mar;161(3):944-8. doi: 10.1128/jb.161.3.944-948.1985.
Abstract
Colicin E1 fragments obtained by genetic manipulations of the ColE1 plasmid were tested for bactericidal activity, binding to bacterial cells, and reactions with a series of anticolicin monoclonal antibodies. Two of the fragments were also tested for ability to form channels in liposomal vesicles. The results are in agreement with studies from chemically and enzymatically derived colicin fragments, assigning the receptor binding activity to the central part of the molecule and the killing activity to a region near the carboxyl terminus.
摘要
通过对ColE1质粒进行基因操作获得的大肠杆菌素E1片段，被测试了杀菌活性、与细菌细胞的结合能力以及与一系列抗大肠杆菌素单克隆抗体的反应。还测试了其中两个片段在脂质体囊泡中形成通道的能力。这些结果与来自化学和酶促衍生的大肠杆菌素片段的研究结果一致，将受体结合活性归属于分子的中央部分，而将杀伤活性归属于羧基末端附近的区域。
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本文引用的文献
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MECHANISM OF ACTION OF COLICINES.大肠杆菌素的作用机制
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On a domain structure of colicin E1. A COOH-terminal peptide fragment active in membrane depolarization.关于大肠杆菌素E1的结构域结构。一个在膜去极化中具有活性的COOH末端肽片段。
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Nucleotide sequence of the structural gene for colicin E1 and predicted structure of the protein.大肠杆菌素E1结构基因的核苷酸序列及蛋白质的预测结构
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Alternative forms of lethality in mitomycin C-induced bacteria carrying ColE1 plasmids.携带ColE1质粒的丝裂霉素C诱导细菌中的致死性替代形式。
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