Suit J L, Fan M L, Kayalar C, Luria S E
J Bacteriol. 1985 Mar;161(3):944-8. doi: 10.1128/jb.161.3.944-948.1985.
Colicin E1 fragments obtained by genetic manipulations of the ColE1 plasmid were tested for bactericidal activity, binding to bacterial cells, and reactions with a series of anticolicin monoclonal antibodies. Two of the fragments were also tested for ability to form channels in liposomal vesicles. The results are in agreement with studies from chemically and enzymatically derived colicin fragments, assigning the receptor binding activity to the central part of the molecule and the killing activity to a region near the carboxyl terminus.
通过对ColE1质粒进行基因操作获得的大肠杆菌素E1片段，被测试了杀菌活性、与细菌细胞的结合能力以及与一系列抗大肠杆菌素单克隆抗体的反应。还测试了其中两个片段在脂质体囊泡中形成通道的能力。这些结果与来自化学和酶促衍生的大肠杆菌素片段的研究结果一致，将受体结合活性归属于分子的中央部分，而将杀伤活性归属于羧基末端附近的区域。
