Dennerlein Kathrin, Jäger Thomas, Göen Thomas, Kilo Sonja, Schaller Karl Heinz, Drexler Hans, Korinth Gintautas
Institute and Out-Patient Clinic of Occupational, Social and Environmental Medicine, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Schillerstrasse 25/29, D-91054 Erlangen, Germany.
Institute and Out-Patient Clinic of Occupational, Social and Environmental Medicine, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Schillerstrasse 25/29, D-91054 Erlangen, Germany.
Toxicol In Vitro. 2015 Aug;29(5):828-33. doi: 10.1016/j.tiv.2015.03.001. Epub 2015 Mar 16.
To reduce the internal exposure, skin decontamination is the most important measure after dermal contact to chemicals. However, no harmonized skin cleaning procedure for experimental ex vivo studies is published. In our study, the impact of two skin cleaning techniques on dermal penetration kinetics and intradermal deposition of 1,4-dioxane, 5% hydrofluoric acid (HF, detected in terms of fluoride ions), and anisole was evaluated to develop a reliable ex vivo skin cleaning method using the diffusion cell technique. After exposure (duration: 3 min (HF); 1h (1,4-dioxane and anisole)) of excised human skin (n=6-8) decontamination was performed by (I) water-soaked cotton swabs or (II) direct application of water on the exposure area. The effect of skin cleaning was investigated by analysing the concentration time course of chemicals in the receptor fluid of diffusion cells and by determining the deposition in skin. Both skin cleaning procedures reduced the amount of fluoride in the skin compartments (p<0.05) and the receptor fluid (p<0.1). However, the effect of cleaning on the dermal absorption of the organic test compounds was not significant. The results demonstrate the suitability of the applied ex vivo protocol for investigating the effectiveness of skin cleaning measures following dermal exposure. In addition, data reveal that the determination of test compounds in both, skin compartments as well as receptor fluid as equivalent for the systemic uptake needs to be considered in studies assessing the effectiveness of skin decontamination procedures.
为减少体内暴露，皮肤去污是皮肤接触化学物质后最重要的措施。然而，目前尚未发布用于实验性离体研究的统一皮肤清洁程序。在我们的研究中，评估了两种皮肤清洁技术对1,4-二氧六环、5%氢氟酸（HF，以氟离子计）和苯甲醚的皮肤渗透动力学及皮内沉积的影响，以使用扩散池技术开发一种可靠的离体皮肤清洁方法。在切除的人体皮肤（n = 6 - 8）暴露（持续时间：3分钟（HF）；1小时（1,4-二氧六环和苯甲醚））后，通过（I）用水浸湿的棉签或（II）直接在暴露区域浇水进行去污。通过分析扩散池受体液中化学物质的浓度时间过程以及测定皮肤中的沉积来研究皮肤清洁的效果。两种皮肤清洁程序均降低了皮肤隔室（p < 0.05）和受体液（p < 0.1）中的氟含量。然而，清洁对有机测试化合物皮肤吸收的影响并不显著。结果表明所应用的离体方案适用于研究皮肤暴露后皮肤清洁措施的有效性。此外，数据显示在评估皮肤去污程序有效性的研究中，需要考虑将皮肤隔室以及受体液中的测试化合物测定作为全身吸收的等效指标。
