Janda W M, Jackson T
J Clin Microbiol. 1985 Feb;21(2):143-5. doi: 10.1128/jcm.21.2.143-145.1985.
Gonodecten (Gd; U.S. Packaging Corp., LaPorte, Ind.) is a commercially available test for the presumptive diagnosis of gonococcal urethritis in men. With this test, urethral discharge is collected on a swab, placed in a plastic tube, and moistened with oxidase reagent. Development of a purple color on the swab within 3 min indicates the presence of gonococci. This system was compared with culture and Gram-stained smear for the diagnosis of gonorrhea. Of 240 specimens tested, 174 were positive with culture, Gram stain, and the Gd test and 48 were negative with all tests. For eight specimens, cultures and smears were positive, but the Gd test was negative. Nine specimens produced positive Gd tests with negative smears and cultures. Only one specimen was positive on culture and with the Gd test and negative on Gram-stained smear. Haemophilus species were isolated from 14 specimens; 5 produced positive Gd test reactions, with gonococci being coisolated from 4. The sensitivity and specificity of the Gd test compared with culture were 95.6 and 84.2%, respectively, whereas the Gram stain was 99.5% sensitive and 100% specific compared with culture. The Gd test may be a useful screening test for the diagnosis of gonorrhea in men with urethral discharge, particularly in settings where a microscope, Gram stain reagents, or technological expertise is not readily available.
淋菌检测试剂（Gd；美国包装公司，印第安纳州拉波特）是一种可用于男性淋菌性尿道炎初步诊断的商用检测方法。使用该检测方法时，用拭子采集尿道分泌物，置于塑料管中，并用氧化酶试剂湿润。3分钟内拭子上出现紫色表明存在淋球菌。将该检测系统与用于淋病诊断的培养法和革兰氏染色涂片法进行了比较。在检测的240份标本中，174份培养、革兰氏染色和Gd检测均呈阳性，48份所有检测均为阴性。有8份标本培养和涂片呈阳性，但Gd检测为阴性。9份标本Gd检测呈阳性，涂片和培养呈阴性。只有1份标本培养和Gd检测呈阳性，革兰氏染色涂片呈阴性。从14份标本中分离出嗜血杆菌属；5份产生阳性Gd检测反应，其中4份同时分离出淋球菌。与培养法相比，Gd检测的敏感性和特异性分别为95.6%和84.2%，而革兰氏染色与培养法相比敏感性为99.5%，特异性为100%。Gd检测可能是一种有用的筛查方法，用于诊断有尿道分泌物的男性淋病，特别是在没有显微镜、革兰氏染色试剂或技术专业人员的情况下。
