Centre for Algae Biomass Research, School of Biotechnology & Health Sciences, Karunya University, Coimbatore, Tamil Nadu 641114, India.
Centre for Algae Biomass Research, School of Biotechnology & Health Sciences, Karunya University, Coimbatore, Tamil Nadu 641114, India.
Ecotoxicol Environ Saf. 2015 Nov;121:258-62. doi: 10.1016/j.ecoenv.2015.03.008. Epub 2015 Mar 16.
Economically viable production facilities for microalgae depend on the optimization of growth parameters with regard to nutrient requirements. Using microalgae to treat industrial effluents containing heavy metals presents an alternative to the current practice of using physical and chemical methods. Present work focuses on the statistical optimization of growth of Chlorococcum humicola to ascertain the maximum production of biomass. Plackett Burman design was carried out to screen the significant variables influencing biomass production. Further, Response Surface Methodology was employed to optimize the effect of inoculum, light intensity and pH on net biomass yield. Optimum conditions for maximum biomass yield were identified to be inoculum at 15%, light intensity to be 1500lx and pH 8.5. Theoretical and predicted values were in agreement and thus the model was found to be significant. Gas chromatography analyses of the FAME derivatives showed a high percentage of saturated fatty acids thereby confirming the biofuel properties of the oil derived from algal biomass.
经济可行的微藻生产设施取决于对营养需求的生长参数进行优化。利用微藻处理含有重金属的工业废水是对当前使用物理和化学方法的一种替代方法。目前的工作重点是对绿球藻生长进行统计优化，以确定生物量的最大产量。采用 Plackett Burman 设计筛选影响生物量生产的显著变量。此外，还采用响应面法来优化接种量、光照强度和 pH 值对净生物量产量的影响。确定最大生物量产量的最佳条件为接种量为 15%，光照强度为 1500lx，pH 值为 8.5。理论值和预测值吻合，因此该模型被认为是有效的。脂肪酸甲酯衍生物的气相色谱分析显示出高比例的饱和脂肪酸，从而证实了从藻类生物质中提取的油具有生物燃料特性。
