Algae Biomass Research Laboratory, Department of Biosciences and Technology, Karunya Institute of Technology and Sciences (Deemed to be University), Coimbatore, Tamil Nadu, 641114, India.
Environ Sci Pollut Res Int. 2019 Dec;26(34):34702-34712. doi: 10.1007/s11356-018-4012-9. Epub 2019 Jan 6.
The approach of combining the microalgae cultivation with wastewater provides a cost-effective and eco-friendly perspective in the production of microalgae-based bio-products. In the present investigation, microalgae Scenedesmus rubescens KACC 2 isolated from catchment region of River Noyyal was found to be efficient in removing nitrogen, phosphorus, and heavy metals from industrial and domestic effluents, which was optimized through central composite design matrix for higher biomass generation. Nutrient requirements for the growth were optimized and evaluated using Plackett-Burman design to check the effect of variables. Three variables, viz., nitrate, phosphate, and inoculums, were found to be significant among the 11 variables tested, and the interaction between these variables and its optimum concentrations were statistically studied using central composite design matrix. The optimized growth conditions of this strain were found to be as nitrate (0.2%), phosphate (0.018%), and inoculums (7.5%). These conditions yielded a higher biomass of 0.73 g/L from the optimized media which was 5.4 times higher than the regular growth media. FT-IR analysis showed the variations in the spectra and also in biomolecular composition with 2-fold increase in the lipid and protein region when grown in optimized culture conditions. Lipid profile showed the presence of saturated and monounsaturated fatty acids in the biomass accepting it as a source of energy feedstock. This study concludes that nitrate, phosphate, and inoculums play a significant role in biomass production of S. rubescens with phycoremediation potential that can be exploited for simultaneous wastewater treatment-coupled biomass production.
将微藻培养与废水处理相结合的方法为基于微藻的生物制品生产提供了具有成本效益和环保的视角。在本研究中，从 Noyyal 河集水区分离出的微藻 Scenedesmus rubescens KACC 2 被发现能够有效地从工业和生活废水中去除氮、磷和重金属，通过中心复合设计矩阵进行优化以获得更高的生物量生成。使用 Plackett-Burman 设计优化和评估营养需求，以检查变量的影响。在测试的 11 个变量中，硝酸盐、磷酸盐和接种物这三个变量被发现是重要的，这些变量之间的相互作用及其最佳浓度使用中心复合设计矩阵进行了统计学研究。发现该菌株的最佳生长条件为硝酸盐（0.2%）、磷酸盐（0.018%）和接种物（7.5%）。在优化的培养基中，这些条件下的生物量达到了 0.73 g/L，比常规生长培养基高出 5.4 倍。FT-IR 分析表明，随着优化培养条件下脂质和蛋白质区域的增加，光谱和生物分子组成也发生了变化。脂质谱显示生物质中存在饱和和单不饱和脂肪酸，使其成为能源原料的来源。这项研究得出结论，硝酸盐、磷酸盐和接种物在 S. rubescens 的生物量生产中起着重要作用，具有光修复潜力，可以用于同时进行废水处理和生物量生产。
