Kim Sung-Hee, Yoon Yeo Cho, Lee Ae Sin, Kang NaNa, Koo JaeHyung, Rhyu Mee-Ra, Park Jae-Ho
Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea.
Department of Brain Science, Daegu Gyeongbuk Institute of Science & Technology, Techno Jungang-daero, Hyeonpung-myeon, Dalseong-Gun, Daegu 711-873, Republic of Korea.
Biochem Biophys Res Commun. 2015 May 1;460(2):404-8. doi: 10.1016/j.bbrc.2015.03.046. Epub 2015 Mar 17.
ORs are ectopically expressed in non-chemosensory tissues including muscle, kidney, and keratinocytes; however, their physiological roles are largely unknown. We found that human olfactory receptor 10J5 (OR10J5) is expressed in the human aorta, coronary artery, and umbilical vein endothelial cells (HUVEC). Lyral induces Ca(2+) and phosphorylation of AKT in HUVEC. A knockdown study showed the inhibition of the lyral-induced Ca(2+) and the phosphorylation AKT and implied that these processes are mediated by OR10J5. In addition, lyral enhanced migration of HUVEC, which were also inhibited by RNAi in a migration assay. In addition, matrigel plug assay showed that lyral enhanced angiogenesis in vivo. Together these data demonstrate the physiological role of OR10J5 in angiogenesis and represent roles of ORs in HUVEC cells.
嗅觉受体（ORs）在包括肌肉、肾脏和角质形成细胞在内的非化学感觉组织中异位表达；然而，它们的生理作用在很大程度上尚不清楚。我们发现人类嗅觉受体10J5（OR10J5）在人主动脉、冠状动脉和脐静脉内皮细胞（HUVEC）中表达。里哪醇可诱导HUVEC中的Ca(2+)和AKT磷酸化。一项敲低研究显示，里哪醇诱导的Ca(2+)和AKT磷酸化受到抑制，这表明这些过程由OR10J5介导。此外，在迁移试验中，里哪醇增强了HUVEC的迁移，而RNAi也抑制了这种迁移。此外，基质胶栓试验表明，里哪醇在体内增强了血管生成。这些数据共同证明了OR10J5在血管生成中的生理作用，并代表了ORs在HUVEC细胞中的作用。
