Immunoenzymatic analysis by monoclonal antibodies of bacterial lipopolysaccharides after transfer to nitrocellulose.

A rapid, sensitive immunoenzymatic technique for the analysis of lipopolysaccharide (LPS) from gram-negative bacteria using monoclonal antibodies is described. After separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the LPS was either stained with silver or electrophoretically transferred to nitrocellulose. After reaction with anti-LPS monoclonal antibodies, the transferred antigens were visualized by reaction with alkaline-phosphatase-labelled anti-mouse antibodies and a substrate containing naphthol phosphoric acid and Fast Red.