Kyriakopoulou Zaharoula, Bletsa Magda, Tsakogiannis Dimitris, Dimitriou Tilemachos G, Amoutzias Grigoris D, Gartzonika Constantina, Levidiotou-Stefanou Stamatina, Markoulatos Panayotis
Department of Biochemistry & Biotechnology, University of Thessaly, 26, Ploutonos & Aeolou str., Larissa 41 221, Greece.
Department of Biochemistry & Biotechnology, University of Thessaly, 26, Ploutonos & Aeolou str., Larissa 41 221, Greece.
Infect Genet Evol. 2015 Jun;32:305-12. doi: 10.1016/j.meegid.2015.03.008. Epub 2015 Mar 17.
Echovirus 3 (E3) serotype has been related with several neurologic diseases, although it constitutes one of the rarely isolated serotypes, with no report of epidemics in Europe. The aim of the present study was to provide insights into the molecular epidemiology and evolution of this enterovirus serotype, while an E3 strain was isolated from sewage in Greece, four years after the initial isolation of the only reported E3 strain in the same geographical region. Phylogenetic analysis of the complete VP1 genomic region of that E3 strain and of those available in GenBank suggested three main genogroups that were further subdivided into seven subgenogroups. Further evolutionary analysis suggested that VP1 genomic region of E3 was dominated by purifying selection, as the vast majority of genetic diversity presumably occurred through synonymous nucleotide substitutions and the substitution rate for complete and partial VP1 sequences was calculated to be 8.13×10(-3) and 7.72×10(-3) substitutions/site/year respectively. The partial VP1 sequence analysis revealed the composite epidemiology of this serotype, as the strains of the three genogroups presented different epidemiological characteristics.
肠道病毒71型（E3）血清型与多种神经系统疾病有关，尽管它是极少分离到的血清型之一，在欧洲尚无疫情报告。本研究的目的是深入了解这种肠道病毒血清型的分子流行病学和进化情况，在希腊同一地理区域首次分离出唯一报告的E3菌株四年后，又从污水中分离出一株E3菌株。对该E3菌株以及GenBank中可用的完整VP1基因组区域进行系统发育分析，结果表明存在三个主要基因群，进一步细分为七个亚基因群。进一步的进化分析表明，E3的VP1基因组区域以纯化选择为主导，因为绝大多数遗传多样性可能是通过同义核苷酸替换发生的，完整和部分VP1序列的替换率分别计算为8.13×10⁻³和7.72×10⁻³替换/位点/年。部分VP1序列分析揭示了该血清型的综合流行病学情况，因为三个基因群的菌株呈现出不同的流行病学特征。
