Fei Jingyi, Singh Digvijay, Zhang Qiucen, Park Seongjin, Balasubramanian Divya, Golding Ido, Vanderpool Carin K, Ha Taekjip
Center for the Physics of Living Cells, Department of Physics, University of Illinois, Urbana, IL, USA.
Center for Biophysics and Computational Biology, University of Illinois, Urbana, IL, USA.
Science. 2015 Mar 20;347(6228):1371-4. doi: 10.1126/science.1258849.
Base-pairing interactions between nucleic acids mediate target recognition in many biological processes. We developed a super-resolution imaging and modeling platform that enabled the in vivo determination of base pairing-mediated target recognition kinetics. We examined a stress-induced bacterial small RNA, SgrS, which induces the degradation of target messenger RNAs (mRNAs). SgrS binds to a primary target mRNA in a reversible and dynamic fashion, and formation of SgrS-mRNA complexes is rate-limiting, dictating the overall regulation efficiency in vivo. Examination of a secondary target indicated that differences in the target search kinetics contribute to setting the regulation priority among different target mRNAs. This super-resolution imaging and analysis approach provides a conceptual framework that can be generalized to other small RNA systems and other target search processes.
核酸之间的碱基配对相互作用在许多生物学过程中介导靶标识别。我们开发了一个超分辨率成像和建模平台，该平台能够在体内确定碱基配对介导的靶标识别动力学。我们研究了一种应激诱导的细菌小RNA，即SgrS，它能诱导靶标信使核糖核酸（mRNA）的降解。SgrS以可逆和动态的方式与主要靶标mRNA结合，SgrS-mRNA复合物的形成是限速步骤，决定了体内的整体调控效率。对次要靶标的研究表明，靶标搜索动力学的差异有助于确定不同靶标mRNA之间的调控优先级。这种超分辨率成像和分析方法提供了一个概念框架，可推广到其他小RNA系统和其他靶标搜索过程。
