Chang L J, Stoltzfus C M
J Virol. 1985 Mar;53(3):969-72. doi: 10.1128/JVI.53.3.969-972.1985.
The cDNAs corresponding to the 5' ends of the mRNAs coding for the envelope protein precursor (gPr92env) of the B77 strain and the transforming protein (pp60src) of the Prague B strain of Rous sarcoma virus were cloned into pBR322, and the nucleotide sequences surrounding the splice junctions were determined. Both mRNAs are products of single splicing events from a common donor splice site at nucleotide 398 from the 5' end of the RNA to acceptor splice sites at nucleotides 5078 and 7054 for the env and src mRNAs, respectively. These results confirm and extend previous conclusions based on peptide mapping and single-strand nuclease mapping. Compared with the sequence of the Prague C genome RNA, the B77 strain contains a 6-nucleotide deletion in the sequence corresponding to the hydrophobic portion of the signal peptide of the envelope protein precursor.
将与劳氏肉瘤病毒B77株包膜蛋白前体（gPr92env）和布拉格B株转化蛋白（pp60src）编码的mRNA 5'端相对应的cDNA克隆到pBR322中，并确定了剪接接头周围的核苷酸序列。两种mRNA均是单次剪接事件的产物，对于env和src mRNA，分别从RNA 5'端第398位核苷酸处的一个共同供体剪接位点剪接到第5078位和第7054位核苷酸处的受体剪接位点。这些结果证实并扩展了基于肽图谱和单链核酸酶图谱得出的先前结论。与布拉格C基因组RNA的序列相比，B77株在与包膜蛋白前体信号肽疏水部分相对应的序列中存在一个6核苷酸缺失。
