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SELEX 研究古菌 FFRPs 的 DNA 结合特异性：2. FL4(pot1613368)。

A SELEX study of the DNA-binding specificity of archaeal FFRPs: 2. FL4 (pot1613368).

机构信息

National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Center 6-10, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

出版信息

Proc Jpn Acad Ser B Phys Biol Sci. 2006 Mar;82(1):33-44. doi: 10.2183/pjab.82.33.

DOI:10.2183/pjab.82.33
PMID:25792768
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4322925/
Abstract

The DNA-binding specificity of a transcription factor, the FFRP FL4 (pot1613368) from Pyrococcus sp. OT3, was studied. Using SELEX (systematic evolution of ligands by exponential environment) experiments, from a set of fragments, ∼150 bps, of the genomic DNA of P. OT3, seven were selected as containing binding sites. Thirteen bases identified as shared by the seven selected fragments with the least mismatches, 2.71 on average, was ATGAA AAAGTCAT. This sequence was closely related with another sequence, ATGAA[AAA/TTT]TTCAT, in the 5-3-5 arrangement, i.e. NANBNCNDNE [AAA/TTT]NENDNCNBNA , where, e.g. NA was the base complementary to NA. The average number of mismatches found between this sequence and the seven fragments was 3.14. A sequence, TTGAA ATT TACAA, resembling the sequence ATGAA[AAA/TTT]TTCAT and also another 5-3-5 sequence, TTGAA[AAA/TTT]TTCAA, was found upstream of the fl4 gene, which is potentially recognized by FL4 for auto-regulation. Thus it is likely that an ideal binding-site of FL4 is ATGAA[AAA/TTT]TTCAT or TTGAA[AAA/TTT]TTCAA. In this abstract, the sequences were highlighted in Italic at 3, and with bold characters at 5 and 5. When two sequences compared were the same at some positions, there they were underlined.

摘要

从 Pyrococcus sp. OT3 中分离的转录因子 FFRP FL4(pot1613368)的 DNA 结合特异性进行了研究。利用 SELEX（指数富集配体系统进化）实验，从 Pyrococcus sp. OT3 基因组 DNA 的一组片段（约 150bp）中，选择了 7 个含有结合位点的片段。这 7 个被选择的片段与另外 7 个片段共有 13 个碱基，平均错配率为 2.71，即 ATGAA AAAGTCAT。该序列与另一个序列 ATGAA[AAA/TTT]TTCAT 密切相关，其排列方式为 5-3-5，即 NANBNCNDNE[AAA/TTT]NENDNCNBNA，其中，例如，NA 是与 NA 互补的碱基。该序列与 7 个片段之间的平均错配数为 3.14。在 fl4 基因的上游，发现了一个与序列 ATGAA[AAA/TTT]TTCAT 相似的序列 TTGAA ATT TACAA，同时也发现了另一个 5-3-5 序列 TTGAA[AAA/TTT]TTCAA，这可能是 FL4 自身调控的潜在识别序列。因此，FL4 的理想结合位点很可能是 ATGAA[AAA/TTT]TTCAT 或 TTGAA[AAA/TTT]TTCAA。在这个摘要中，序列在 3 处用斜体突出显示，在 5 和 5 处用粗体显示。当比较的两个序列在某些位置相同时，这些位置用下划线表示。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/712d/4322925/3b11812d73c6/82_33f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/712d/4322925/3ef991982c09/82_33f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/712d/4322925/56bbb1196fc2/82_33f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/712d/4322925/e9e74010de9e/82_33f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/712d/4322925/23af390cdbb3/82_33f4a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/712d/4322925/3b11812d73c6/82_33f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/712d/4322925/3ef991982c09/82_33f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/712d/4322925/56bbb1196fc2/82_33f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/712d/4322925/e9e74010de9e/82_33f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/712d/4322925/23af390cdbb3/82_33f4a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/712d/4322925/3b11812d73c6/82_33f5.jpg

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本文引用的文献

1
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FEMS Microbiol Rev. 2006 Jan;30(1):89-108. doi: 10.1111/j.1574-6976.2005.00005.x.
2
The archaeal feast/famine regulatory protein: potential roles of its assembly forms for regulating transcription.古菌的 feast/famine 调控蛋白：其组装形式在转录调控中的潜在作用
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2840-5. doi: 10.1073/pnas.0400109101. Epub 2004 Feb 19.
3
Crystallization and secondary-structure determination of a protein of the Lrp/AsnC family from a hyperthermophilic archaeon.
Nucleic Acids Res. 2006 Mar 9;34(5):1439-49. doi: 10.1093/nar/gkl009. Print 2006.
嗜热古菌Lrp/AsnC家族一种蛋白质的结晶及二级结构测定
Acta Crystallogr D Biol Crystallogr. 2001 Mar;57(Pt 3):469-71. doi: 10.1107/s0907444900020369.
4
Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3.嗜热古细菌嗜热栖热菌OT3基因组的完整序列和基因组织
DNA Res. 1998 Apr 30;5(2):55-76. doi: 10.1093/dnares/5.2.55.
5
New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.对含有相邻组氨酸残基的蛋白质和肽具有选择性的新型金属螯合吸附剂。
J Chromatogr. 1987 Dec 18;411:177-84. doi: 10.1016/s0021-9673(00)93969-4.
6
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。
Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.
7
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.指数富集的配体系统进化：噬菌体T4 DNA聚合酶的RNA配体
Science. 1990 Aug 3;249(4968):505-10. doi: 10.1126/science.2200121.