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基于细胞成像的多参数分析的中药注射液安全性评价显示，线粒体功能在肝毒性中起关键作用。

Safety evaluation of chinese medicine injections with a cell imaging-based multiparametric assay revealed a critical involvement of mitochondrial function in hepatotoxicity.

机构信息

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China ; Research and Development Center of TCM, Tianjin International Joint Academy of Biotechnology & Medicine, 220 Dongting Road, TEDA, Tianjin 300457, China.

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China.

出版信息

Evid Based Complement Alternat Med. 2015;2015:379586. doi: 10.1155/2015/379586. Epub 2015 Feb 22.

DOI:10.1155/2015/379586
PMID:25792997
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4352439/
Abstract

The safety of herbal medicine products has been a widespread concern due to their complex chemical nature and lack of proper evaluation methods. We have adapted a sensitive and reproducible multiparametric cell-based high-content analysis assay to evaluate the hepatic-safety of four Chinese medicine injections and validated it with classical animal-based toxicity assays. Our results suggested that the reported hepatotoxicity by one of the drugs, Fufangkushen injection, could be attributed at least in part to the interference of mitochondrial function in human HepG2 cells by some of its constituents. This method should be useful for both preclinical screen in a drug discovery program and postclinical evaluation of herbal medicine preparations.

摘要

由于草药产品的复杂化学性质和缺乏适当的评估方法，其安全性一直是一个广泛关注的问题。我们采用了一种敏感且可重复的基于多参数细胞的高通量分析测定法来评估四种中药注射液的肝安全性，并通过经典的基于动物的毒性测定法对其进行了验证。我们的结果表明，其中一种药物——复方苦参注射液所报告的肝毒性至少部分归因于其某些成分对人 HepG2 细胞中线粒体功能的干扰。该方法对于药物发现计划中的临床前筛选和草药制剂的临床后评估都应该是有用的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/99c7111bf18d/ECAM2015-379586.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/925dbde86968/ECAM2015-379586.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/3e8509d73df0/ECAM2015-379586.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/c27a363d6645/ECAM2015-379586.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/3e1c4dde3173/ECAM2015-379586.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/3b8893b34488/ECAM2015-379586.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/9475f8be1c26/ECAM2015-379586.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/99c7111bf18d/ECAM2015-379586.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/925dbde86968/ECAM2015-379586.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/3e8509d73df0/ECAM2015-379586.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/c27a363d6645/ECAM2015-379586.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/3e1c4dde3173/ECAM2015-379586.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/3b8893b34488/ECAM2015-379586.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/9475f8be1c26/ECAM2015-379586.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e890/4352439/99c7111bf18d/ECAM2015-379586.007.jpg

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