Department of Biomedical Engineering, University of Rochester School of Medicine and Dentistry, Rochester, NY 14627, USA.
Biotechniques. 2011 Feb;50(2):98-115. doi: 10.2144/000113610.
Fluorescent probes for monitoring mitochondrial membrane potential are frequently used for assessing mitochondrial function, particularly in the context of cell fate determination in biological and biomedical research. However, valid interpretation of results obtained with such probes requires careful consideration of numerous controls, as well as possible effects of non-protonic charges on dye behavior. In this context, we provide an overview of some of the important technical considerations, controls, and parallel complementary assays that can be employed to help ensure appropriate interpretation of results, thus providing a practical usage guide for monitoring mitochondrial membrane potentials with cationic probes. In total, this review will help illustrate both the strengths and potential pitfalls of common mitochondrial membrane potential dyes, and highlight best-usage approaches for their efficacious application in life sciences research.
用于监测线粒体膜电位的荧光探针常用于评估线粒体功能，特别是在生物学和生物医学研究中细胞命运决定的背景下。然而，使用此类探针获得的结果的有效解释需要仔细考虑许多对照，以及非质子电荷对染料行为的可能影响。在这种情况下，我们提供了一些重要技术考虑因素、对照和并行互补测定的概述，这些可以用来帮助确保结果的适当解释，从而为使用阳离子探针监测线粒体膜电位提供实用的使用指南。总的来说，这篇综述将有助于说明常见线粒体膜电位染料的优缺点，并强调在生命科学研究中有效应用它们的最佳方法。
