1] Tissue Repair Lab, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. [2] Research Center for Neural Repair, University of Tehran, Tehran, Iran.
Department of Orthopedic and Trauma Surgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Lab Anim (NY). 2015 Apr;44(4):141-5. doi: 10.1038/laban.711.
Denervation of the hind limb is a technique used to study peripheral nerve regeneration. Autotomy or autophagia is an undesirable response to denervation in such studies. Application of a commercially available lotion used to deter nail biting in humans reduced autotomy in rats after denervation but did not completely prevent it. In this study, this authors evaluated the application of picric acid to prevent autotomy in rats in peripheral nerve experiments. They carried out sciatic nerve transection in 41 adult female Wistar rats and then applied either bite-deterrent lotion (n = 26) or saturated picric acid solution (n = 15) topically to the affected hind limb immediately after surgery and every day for 1 month. Autotomy scores were lower for rats treated with picric acid than for rats treated with bite-deterrent lotion 1 week and 2 weeks after surgery but were not different between the two groups 4 weeks after surgery. The authors conclude that application of picric acid could be used as an alternative strategy to prevent autotomy in peripheral nerve studies.
去神经支配后肢是一种用于研究周围神经再生的技术。在这些研究中，自截或自噬是去神经支配后一种不理想的反应。应用一种市售的用于防止人类咬指甲的乳液可以减少去神经支配后大鼠的自截现象，但不能完全预防。在这项研究中，作者评估了应用苦味酸来防止周围神经实验中大鼠的自截现象。他们对 41 只成年雌性 Wistar 大鼠进行坐骨神经横断术，然后在手术后立即和每天用咬避剂（n = 26）或饱和苦味酸溶液（n = 15）局部应用于受影响的后肢，持续 1 个月。与使用咬避剂的大鼠相比，使用苦味酸的大鼠在手术后 1 周和 2 周的自截评分较低，但在手术后 4 周时两组之间没有差异。作者得出结论，应用苦味酸可以作为防止周围神经研究中自截的一种替代策略。
